PMID- 26845570
OWN - NLM
STAT- MEDLINE
DCOM- 20160712
LR  - 20240327
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - Extracellular Vesicles from BOEC in In Vitro Embryo Development and Quality.
PG  - e0148083
LID - 10.1371/journal.pone.0148083 [doi]
LID - e0148083
AB  - To evaluate the effect of conditioned media (CM) and Extracellular Vesicles (EVs) 
      derived from bovine oviduct epithelial cell (BOEC) lines on the developmental 
      capacity of bovine zygotes and the quality of embryos produced in vitro, 
      presumptive zygotes were cultured under specific conditions. In experiment 1, 
      zygotes were cultured either on monolayers from BOEC extended culture (E), 
      together with fresh BOEC suspension cells, or with BOEC-CM from fresh or 
      E-monolayers. In experiment 2, EVs were isolated from BOEC-CM and characterized 
      (150-200 nm) by Nanosight(R) and electron microscopy. Zygotes were cultured in the 
      presence of 3x10(5) EVs/mL, 1.5x10(5) EVs/mL or 7.5x10(4) EVs/mL of fresh or 
      frozen BOEC-EVs. In experiment 3, zygotes were cultured in absence of FCS but 
      with EVs from BOEC-E that had been cultured in different culture media. In 
      experiment 4, zygotes were cultured in SOF+5% normal-FCS, or EV-depleted-FCS. In 
      all cases, cleavage rate (Day 2) and blastocyst development (Day 7-9) was 
      assessed. Blastocysts on Days 7/8 were used for quality evaluation through 
      differential cell count, cryotolerance and gene expression patterns. No 
      differences were found among all FCS-containing groups in cleavage rate or 
      blastocyst yield. However, embryos derived from BOEC-CM had more trophectoderm 
      cells, while embryos derived from BOEC-EVs, both fresh and frozen, has more 
      trophectoderm and total cells. More embryos survived vitrification in the BOEC-CM 
      and BOEC-EV groups. In contrast, more embryos survived in the EV-depleted-FCS 
      than in normal-FCS group. Gene expression patterns were modified for PAG1 for 
      embryos cultured with EVs in the presence of FCS and for IFN-T, PLAC8, PAG1, 
      CX43, and GAPDH in the absence of FCS. In conclusion, EVs from FCS have a 
      deleterious effect on embryo quality. BOEC-CM and EVs during in vitro culture had 
      a positive effect on the quality of in vitro produced bovine embryos, suggesting 
      that EVs have functional communication between the oviduct and the embryo in the 
      early stages of development.
FAU - Lopera-Vasquez, Ricaurte
AU  - Lopera-Vasquez R
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Hamdi, Meriem
AU  - Hamdi M
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Fernandez-Fuertes, Beatriz
AU  - Fernandez-Fuertes B
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Maillo, Veronica
AU  - Maillo V
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Beltran-Brena, Paula
AU  - Beltran-Brena P
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Calle, Alexandra
AU  - Calle A
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Redruello, Alberto
AU  - Redruello A
AD  - Hospital Universitario Santa Cristina, Instituto de Investigaciones Sanitarias 
      Princesa (IIs-IP), Madrid, Spain.
FAU - Lopez-Martin, Soraya
AU  - Lopez-Martin S
AD  - Hospital Universitario Santa Cristina, Instituto de Investigaciones Sanitarias 
      Princesa (IIs-IP), Madrid, Spain.
FAU - Gutierrez-Adan, Alfonso
AU  - Gutierrez-Adan A
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Yanez-Mo, Maria
AU  - Yanez-Mo M
AD  - Hospital Universitario Santa Cristina, Instituto de Investigaciones Sanitarias 
      Princesa (IIs-IP), Madrid, Spain.
AD  - Departamento de Biologia Molecular, UAM/CBM-SO, Madrid, Spain.
FAU - Ramirez, Miguel Angel
AU  - Ramirez MA
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
FAU - Rizos, Dimitrios
AU  - Rizos D
AD  - Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y 
      Tecnologia Agraria y Alimentaria, INIA, Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160204
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blastocyst/cytology/drug effects
MH  - Cattle
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Coculture Techniques
MH  - Culture Media, Conditioned/pharmacology
MH  - Embryo Culture Techniques
MH  - *Embryonic Development
MH  - Epigenesis, Genetic
MH  - Epithelial Cells/*metabolism
MH  - Extracellular Vesicles/*metabolism
MH  - Fatty Acids/metabolism
MH  - Female
MH  - In Vitro Techniques
MH  - Oviducts/*cytology/*metabolism
MH  - RNA, Messenger/genetics
PMC - PMC4742056
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/06 06:00
MHDA- 2016/07/13 06:00
PMCR- 2016/02/04
CRDT- 2016/02/05 06:00
PHST- 2015/06/29 00:00 [received]
PHST- 2016/01/12 00:00 [accepted]
PHST- 2016/02/05 06:00 [entrez]
PHST- 2016/02/06 06:00 [pubmed]
PHST- 2016/07/13 06:00 [medline]
PHST- 2016/02/04 00:00 [pmc-release]
AID - PONE-D-15-27691 [pii]
AID - 10.1371/journal.pone.0148083 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 4;11(2):e0148083. doi: 10.1371/journal.pone.0148083. 
      eCollection 2016.