PMID- 26846479
OWN - NLM
STAT- MEDLINE
DCOM- 20161226
LR  - 20161230
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 48
IP  - 4
DP  - 2016 Apr
TI  - Lentiviral-mediated overexpression of long non-coding RNA GAS5 reduces invasion 
      by mediating MMP2 expression and activity in human melanoma cells.
PG  - 1509-18
LID - 10.3892/ijo.2016.3377 [doi]
AB  - The present study evaluated the effects of long non-coding RNA GAS5 on the 
      migration and invasion of melanoma cells. Using the SK-Mel‑110 melanoma cell 
      line, we stably expressed GAS5, visualized the distribution of GAS5 by RNA 
      fluorescence in situ hybridization (FISH) and examined changes in cell migration 
      and invasion with Transwell assays. In GAS5 overexpressed SK-Mel‑110 cells, 
      migrated and invaded cells decreased by 65.3 and 55.6%, respectively. Moreover, 
      the MMP2 protein level, and its activity was downregulated by 67.9 and 15.8%, 
      respectively. Overexpressing lncRNA GAS5 inhibited the migration and invasion 
      ability of melanoma SK-Mel‑110 cells, partially by decreasing the MMP2 expression 
      and its activity. This study is the first to reveal a potential relationship 
      between lncRNA GAS5 and the migration and invasion of melanoma.
FAU - Chen, Long
AU  - Chen L
AD  - Department of Biochemistry and Molecular Biology of Kunming Medical University, 
      Yunnan, P.R. China.
FAU - Yang, Huixin
AU  - Yang H
AD  - Department of Biochemistry and Molecular Biology of Kunming Medical University, 
      Yunnan, P.R. China.
FAU - Xiao, Yanbin
AU  - Xiao Y
AD  - Department of Orthopaedic Surgery, The Third Affiliated Hospital of Kunming 
      Medical University, Yunnan, P.R. China.
FAU - Tang, Xiaoxia
AU  - Tang X
AD  - Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical 
      University, Yunnan, P.R. China.
FAU - Li, Yuqian
AU  - Li Y
AD  - Department of Biochemistry and Molecular Biology of Kunming Medical University, 
      Yunnan, P.R. China.
FAU - Han, Qiaoqiao
AU  - Han Q
AD  - Department of Biochemistry and Molecular Biology of Kunming Medical University, 
      Yunnan, P.R. China.
FAU - Fu, Junping
AU  - Fu J
AD  - Department of Orthopaedic Surgery, The Third Affiliated Hospital of Kunming 
      Medical University, Yunnan, P.R. China.
FAU - Yang, Yuye
AU  - Yang Y
AD  - Department of Biochemistry and Molecular Biology of Kunming Medical University, 
      Yunnan, P.R. China.
FAU - Zhu, Yuechun
AU  - Zhu Y
AD  - Department of Biochemistry and Molecular Biology of Kunming Medical University, 
      Yunnan, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160204
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (GAS5 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Nucleus/genetics
MH  - Cytoplasm/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lentivirus/genetics
MH  - Matrix Metalloproteinase 2/*genetics/*metabolism
MH  - Melanoma/genetics/*pathology
MH  - Neoplasm Invasiveness
MH  - RNA, Long Noncoding/chemistry/*genetics
EDAT- 2016/02/06 06:00
MHDA- 2016/12/27 06:00
CRDT- 2016/02/06 06:00
PHST- 2015/12/01 00:00 [received]
PHST- 2016/01/07 00:00 [accepted]
PHST- 2016/02/06 06:00 [entrez]
PHST- 2016/02/06 06:00 [pubmed]
PHST- 2016/12/27 06:00 [medline]
AID - 10.3892/ijo.2016.3377 [doi]
PST - ppublish
SO  - Int J Oncol. 2016 Apr;48(4):1509-18. doi: 10.3892/ijo.2016.3377. Epub 2016 Feb 4.