PMID- 26846721 OWN - NLM STAT- MEDLINE DCOM- 20161031 LR - 20181202 IS - 1567-2387 (Electronic) IS - 1567-2379 (Linking) VI - 47 IP - 2 DP - 2016 Apr TI - The angiogenic variation of skeletal site-specific human BMSCs from same alveolar cleft patients: a comparative study. PG - 153-68 LID - 10.1007/s10735-016-9662-7 [doi] AB - Tissue engineering strategies hold great potential for alveolar cleft reconstruction. Bone marrow stromal cells (BMSCs) from iliac crest and craniofacial regions are candidate seeding cells with site-specific characteristics and bone-repairing properties. Craniofacial BMSCs seem to possess stronger multipotency and osteogenic capabilities than BMSCs isolated from iliac crest. However, the angiogenic capabilities of these two type cell is rarely reported. We obtained human BMSCs (hBMSCs) of maxilla (M-hBMSCs) and iliac crest (I-hBMSCs) from same alveolar cleft patients to investigate the agiogenic variations using co-culture system with human umbilical vein endothelial cells (HUVECs). From in vitro comparison, M-hBMSCs allowed HUVECs to form more tube-like structures and sprouting angiogenesis by tube formation assays and 3D fibrin vasculogenic assay, respectively. By transplantation in vivo, M-hBMSCs enhanced larger size vessel like structures distributed the entire implants compared with I-hBMSCs. Western blotting was used to assess the angiogenesis related factors including hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). The results showed a significant higher expression of bFGF protein in M-hBMSCs and HUVECs co-culture system both in vitro and in vivo. As bFGF could promote migration and proliferation of endothelial cells, scratch wound healing and transwell migration assays were performed as well as MTT assays and cell cycle analysis. The data suggested the effect of M-hBMSCs on HUVECs was stronger than I-hBMSCs. Taken together, these results indicated that craniofacial BMSCs seemed to have greater angiogenesis capability than iliac crest BMSCs and this might be associated with the different levels of bFGF protein expression in co-culture system. FAU - Du, Yifei AU - Du Y AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Jiang, Fei AU - Jiang F AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Liang, Yi AU - Liang Y AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Wang, Yuli AU - Wang Y AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Zhou, Weina AU - Zhou W AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Polyclinic Dentistry, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Pan, Yongchu AU - Pan Y AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Orthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Xue, Mingfei AU - Xue M AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Peng, Yan AU - Peng Y AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Yuan, Huan AU - Yuan H AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. FAU - Chen, Ning AU - Chen N AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. danxiangren2006@163.com. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. danxiangren2006@163.com. FAU - Jiang, Hongbing AU - Jiang H AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. jhbcd@sina.com. AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. jhbcd@sina.com. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160204 PL - Netherlands TA - J Mol Histol JT - Journal of molecular histology JID - 101193653 RN - 103107-01-3 (Fibroblast Growth Factor 2) SB - IM MH - Adipogenesis MH - Adolescent MH - Bone and Bones/*cytology MH - Cell Differentiation MH - Cell Movement MH - Cell Proliferation MH - Child MH - Cleft Lip/*pathology MH - Coculture Techniques MH - Female MH - Fibroblast Growth Factor 2/metabolism MH - Human Umbilical Vein Endothelial Cells/cytology MH - Humans MH - Male MH - Maxilla/cytology MH - Mesenchymal Stem Cells/*cytology MH - *Neovascularization, Physiologic MH - Osteogenesis OTO - NOTNLM OT - Alveolar cleft graft OT - Angiogenesis OT - Basic fibroblast growth factor (bFGF) OT - Co-culture OT - Human bone marrow mesenchymal stem cells (hBMSCs) OT - Site-specific characteristic EDAT- 2016/02/06 06:00 MHDA- 2016/11/01 06:00 CRDT- 2016/02/06 06:00 PHST- 2015/12/01 00:00 [received] PHST- 2016/01/22 00:00 [accepted] PHST- 2016/02/06 06:00 [entrez] PHST- 2016/02/06 06:00 [pubmed] PHST- 2016/11/01 06:00 [medline] AID - 10.1007/s10735-016-9662-7 [pii] AID - 10.1007/s10735-016-9662-7 [doi] PST - ppublish SO - J Mol Histol. 2016 Apr;47(2):153-68. doi: 10.1007/s10735-016-9662-7. Epub 2016 Feb 4.