PMID- 26847293
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20181202
IS  - 1976-2437 (Electronic)
IS  - 0513-5796 (Print)
IS  - 0513-5796 (Linking)
VI  - 57
IP  - 2
DP  - 2016 Mar
TI  - Standardization of Weed Pollen Extracts, Japanese Hop and Mugwort, in Korea.
PG  - 399-406
LID - 10.3349/ymj.2016.57.2.399 [doi]
AB  - PURPOSE: Japanese hop (Humulus spp.) and mugwort (Artemisia spp.) are notable 
      causes of autumn pollinosis in East Asia. However, Japanese hop and mugwort 
      pollen extracts, which are widely used for the diagnosis, have not been 
      standardized. This study was performed to standardize Japanese hop and mugwort 
      pollen extracts. MATERIALS AND METHODS: Allergen extracts were prepared in a 
      standardized way using locally collected Humulus japonicus and purchased 
      Artemisia vulgaris pollens. The immunoglobulin E (IgE) reactivities of prepared 
      extracts were compared with commercial extracts via IgE immunoblotting and 
      inhibition analyses. Intradermal skin tests were performed to determine the 
      bioequivalent allergy unit (BAU). RESULTS: The IgE reactive components of the 
      extracts via IgE immunoblotting were similar to those of commercial extracts. A 
      11-kDa allergen showed the strongest IgE reactivity in Japanese hop, as did a 
      28-kDa allergen in mugwort pollen extracts. Allergenic potencies of the 
      investigatory Japanese hop and mugwort extracts were essentially 
      indistinguishable from the commercial ones. Sums of erythema of 50 mm by the 
      intradermal skin test (SigmaED50) were calculated to be 14.4th and 13.6th three-fold 
      dilutions for Japanese hop and mugwort extracts, respectively. Therefore, the 
      allergenic activity of the prepared extracts was 90827.4 BAU/mg for Japanese hop 
      and 34412 BAU/mg for mugwort. CONCLUSION: We produced Japanese hop and mugwort 
      pollen extracts using a standardized method. Standardized Japanese hop and 
      mugwort pollen extracts will facilitate the production of improved diagnostic and 
      immunotherapeutic reagents.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Son, Mina
AU  - Son M
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Choi, Soo Young
AU  - Choi SY
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Park, Kyung Hee
AU  - Park KH
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Park, Hye Jung
AU  - Park HJ
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Hong, Chein Soo
AU  - Hong CS
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Lee, Jae Hyun
AU  - Lee JH
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Park, Jung Won
AU  - Park JW
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea. parkjw@yuhs.ac.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Yonsei Med J
JT  - Yonsei medical journal
JID - 0414003
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*analysis/*immunology
MH  - Antibody Specificity
MH  - *Artemisia
MH  - Bronchial Hyperreactivity/blood/immunology
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/blood/*immunology
MH  - Pollen/*chemistry/*immunology
MH  - Reference Standards
MH  - Republic of Korea
MH  - Rhinitis, Allergic, Seasonal
PMC - PMC4740533
OTO - NOTNLM
OT  - Allergen standardization
OT  - Japanese hop
OT  - mugwort
COIS- The authors have no financial conflicts of interest.
EDAT- 2016/02/06 06:00
MHDA- 2016/11/01 06:00
PMCR- 2016/03/01
CRDT- 2016/02/06 06:00
PHST- 2015/04/01 00:00 [received]
PHST- 2015/07/24 00:00 [revised]
PHST- 2015/08/10 00:00 [accepted]
PHST- 2016/02/06 06:00 [entrez]
PHST- 2016/02/06 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - 57.399 [pii]
AID - 10.3349/ymj.2016.57.2.399 [doi]
PST - ppublish
SO  - Yonsei Med J. 2016 Mar;57(2):399-406. doi: 10.3349/ymj.2016.57.2.399.