PMID- 26848638
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1533-4023 (Electronic)
IS  - 0160-2446 (Linking)
VI  - 67
IP  - 2
DP  - 2016 Feb
TI  - Loss of Anticontractile Effect of Perivascular Adipose Tissue on Pregnant Rats: A 
      Potential Role of Tumor Necrosis Factor-alpha.
PG  - 145-51
LID - 10.1097/FJC.0000000000000326 [doi]
AB  - The present investigation examined the effect of pregnancy on the anticontractile 
      effect of perivascular adipose tissue (PVAT) on the rat. Ring segments of the 
      aorta, with and without PVAT, were set up in organ baths for isometric tension 
      recording. In both groups, concentration-response curves to 5-hydroxytryptamine 
      (5-HT) were displaced to the right with a reduction of the maximum response in 
      aorta segments with PVAT. The anticontractile effect of PVAT was attenuated on 
      segments from pregnant rats. 4-Aminopyridine (4-AP), an inhibitor of 
      voltage-gated potassium (Kv) channels, enhanced 5-HT-induced contractions of 
      aorta segments from pregnant and nonpregnant rats only when PVAT was attached. 
      There was no difference in the effect of 4-aminopyridine on 5-HT-induced 
      contractions of aorta segments with PVAT from pregnant and nonpregnant rats. 
      There was also no significant difference in the expression of Kv7.4 channels in 
      aorta segments (with PVAT) between pregnant and nonpregnant rats. Tumor necrosis 
      factor-alpha (TNF-alpha) was detected in PVAT from pregnant and nonpregnant rats. The 
      level of TNF-alpha was significantly greater in PVAT from pregnant rats. Treatment of 
      pregnant rats with pentoxyphyline significantly reduced the level of TNF-alpha in the 
      PVAT and restored the anticontractile effect of PVAT on aorta segments from 
      pregnant rats. Finally, TNF-alpha (10 ng/mL) potentiated 5-HT-induced contractions of 
      PVAT-containing pregnant rat aorta. These results would suggest that the loss of 
      anticontractile effect of PVAT in pregnant rat aorta could be due to enhanced 
      production of TNF-alpha in the PVAT in these rats.
FAU - Al-Jarallah, Aishah
AU  - Al-Jarallah A
AD  - Departments of *Biochemistry; and daggerPharmacology and Toxicology, Faculty of 
      Medicine, Kuwait University, Kuwait.
FAU - Oriowo, Mabayoje A
AU  - Oriowo MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cardiovasc Pharmacol
JT  - Journal of cardiovascular pharmacology
JID - 7902492
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 333DO1RDJY (Serotonin)
RN  - BH3B64OKL9 (4-Aminopyridine)
SB  - IM
MH  - 4-Aminopyridine/pharmacology
MH  - Adipose Tissue/drug effects/*physiology
MH  - Animals
MH  - Aorta, Thoracic/drug effects/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Pregnancy/drug effects/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/pharmacology
MH  - Tumor Necrosis Factor-alpha/*physiology
MH  - Vasoconstriction/drug effects/*physiology
EDAT- 2016/02/06 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/06 06:00
PHST- 2016/02/06 06:00 [entrez]
PHST- 2016/02/06 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 00005344-201602000-00006 [pii]
AID - 10.1097/FJC.0000000000000326 [doi]
PST - ppublish
SO  - J Cardiovasc Pharmacol. 2016 Feb;67(2):145-51. doi: 10.1097/FJC.0000000000000326.