PMID- 26849392
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160708
LR  - 20160302
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 8
DP  - 2016 Mar 2
TI  - Highly Sensitive and Selective Surface-Enhanced Raman Spectroscopy Label-free 
      Detection of 3,3',4,4'-Tetrachlorobiphenyl Using DNA Aptamer-Modified Ag-Nanorod 
      Arrays.
PG  - 5723-8
LID - 10.1021/acsami.5b12866 [doi]
AB  - An improved label-free approach for highly sensitive and selective detection of 
      3,3',4,4'-tetrachlorobiphenyl (PCB-77), a type of polychlorinated biphenyl, via 
      surface-enhanced Raman spectroscopy (SERS) using DNA aptamer-modified Ag-nanorod 
      arrays as the effective substrate is reported. The devised system consists of 
      Ag-nanorod (Ag-NR) arrays with the PCB-77 binding aptamers anchored covalently to 
      the Ag surfaces through a thiol linker. The aptamers are made of single-stranded 
      DNA (ssDNA) oligomers, with one end standing on the Ag surface, and upon 
      conjugation with PCB-77, the ssDNA molecules can change their conformation to 
      hairpin loops, so that the Raman intensity of guanines at the other end of the 
      DNA strand increases accordingly. As such, the intensity ratio I(656 
      cm(-1))/I(733 cm(-1)) increases concomitantly with the increase of the 
      concentration of PCB-77, making the quantitative evaluation of trace amounts of 
      PCB-77 attainable. Moreover, it is found that the DNA aptamer-based Ag-NR arrays 
      can be more responsive with a lower and optimal density of the DNA molecules 
      modified on the substrate surface, and the best sensitivity for detection of 
      PCB-77 can be achieved with the lower detection limit approaching 3.3 x 10(-8) M. 
      This work therefore demonstrates that the design of aptamer-modified Ag-NRs can 
      be used as a practically promising SERS substrate for label-free trace detection 
      of persistent organic pollutants (POPs) in the environment.
FAU - Sun, Kexi
AU  - Sun K
FAU - Huang, Qing
AU  - Huang Q
AD  - University of Science and Technology of China , Hefei, Anhui 230026, People's 
      Republic of China.
FAU - Meng, Guowen
AU  - Meng G
AD  - University of Science and Technology of China , Hefei, Anhui 230026, People's 
      Republic of China.
FAU - Lu, Yilin
AU  - Lu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160217
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
OTO - NOTNLM
OT  - Ag-nanorod arrays
OT  - aptamers
OT  - label-free detection
OT  - polychlorinated biphenyls
OT  - surface-enhanced Raman spectroscopy (SERS)
EDAT- 2016/02/06 06:00
MHDA- 2016/02/06 06:01
CRDT- 2016/02/06 06:00
PHST- 2016/02/06 06:00 [entrez]
PHST- 2016/02/06 06:00 [pubmed]
PHST- 2016/02/06 06:01 [medline]
AID - 10.1021/acsami.5b12866 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 Mar 2;8(8):5723-8. doi: 10.1021/acsami.5b12866. 
      Epub 2016 Feb 17.