PMID- 26850275
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20220331
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 170
IP  - 4
DP  - 2016 Apr
TI  - Salt Stress and Ethylene Antagonistically Regulate Nucleocytoplasmic Partitioning 
      of COP1 to Control Seed Germination.
PG  - 2340-50
LID - 10.1104/pp.15.01724 [doi]
AB  - Seed germination, a critical stage initiating the life cycle of a plant, is 
      severely affected by salt stress. However, the underlying mechanism of salt 
      inhibition of seed germination (SSG) is unclear. Here, we report that the 
      Arabidopsis (Arabidopsis thaliana) CONSTITUTIVE PHOTOMORPHOGENESIS1 (COP1) 
      counteracts SSG Genetic assays provide evidence that SSG in loss of function of 
      the COP1 mutant was stronger than this in the wild type. A GUS-COP1 fusion was 
      constitutively localized to the nucleus in radicle cells. Salt treatment caused 
      COP1 to be retained in the cytosol, but the addition of ethylene precursor 
      1-aminocyclopropane-1-carboxylate had the reverse effect on the translocation of 
      COP1 to the nucleus, revealing that ethylene and salt exert opposite regulatory 
      effects on the localization of COP1 in germinating seeds. However, loss of 
      function of the ETHYLENE INSENSITIVE3 (EIN3) mutant impaired the 
      ethylene-mediated rescue of the salt restriction of COP1 to the nucleus. Further 
      research showed that the interaction between COP1 and LONG HYPOCOTYL5 (HY5) had a 
      role in SSG Correspondingly, SSG in loss of function of HY5 was suppressed. 
      Biochemical detection showed that salt promoted the stabilization of HY5, whereas 
      ethylene restricted its accumulation. Furthermore, salt treatment stimulated and 
      ethylene suppressed transcription of ABA INSENSITIVE5 (ABI5), which was directly 
      transcriptionally regulated by HY5. Together, our results reveal that salt stress 
      and ethylene antagonistically regulate nucleocytoplasmic partitioning of COP1, 
      thereby controlling Arabidopsis seed germination via the COP1-mediated 
      down-regulation of HY5 and ABI5. These findings enhance our understanding of the 
      stress response and have great potential for application in agricultural 
      production.
CI  - (c) 2016 American Society of Plant Biologists. All Rights Reserved.
FAU - Yu, Yanwen
AU  - Yu Y
AUID- ORCID: 0000-0002-5469-500X
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.).
FAU - Wang, Juan
AU  - Wang J
AUID- ORCID: 0000-0003-1636-4450
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.).
FAU - Shi, Hui
AU  - Shi H
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.).
FAU - Gu, Juntao
AU  - Gu J
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.).
FAU - Dong, Jingao
AU  - Dong J
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.).
FAU - Deng, Xing Wang
AU  - Deng XW
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.) 
      deng@pku.edu.cn rfhuang@caas.cn.
FAU - Huang, Rongfeng
AU  - Huang R
AUID- ORCID: 0000-0002-3039-0850
AD  - College of Life Sciences, Hebei Agricultural University, Baoding 071001, China 
      (Y.Y., J.G., J.D.); Biotechnology Research Institute, Chinese Academy of 
      Agricultural Sciences, Beijing 100081, China (Y.Y., J.W., R.H.); School of 
      Advanced Agricultural Sciences and School of Life Sciences, Peking University, 
      Beijing 100871, China (H.S., X.W.D.); and National Key Facility of Crop Gene 
      Resources and Genetic Improvement, Beijing 100081, China (J.W., R.H.) 
      deng@pku.edu.cn rfhuang@caas.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160205
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Ethylenes)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 91GW059KN7 (ethylene)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/drug effects/physiology
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Cell Nucleus/drug effects/*metabolism
MH  - Cytosol/drug effects/metabolism
MH  - Ethylenes/*pharmacology
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Germination/*drug effects
MH  - Models, Biological
MH  - Mutation/genetics
MH  - Protein Stability/drug effects
MH  - Protein Transport/drug effects
MH  - Seeds/drug effects/genetics/*growth & development
MH  - Sodium Chloride/*pharmacology
MH  - Stress, Physiological/*drug effects/genetics
MH  - Transcription, Genetic/drug effects
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
PMC - PMC4825130
EDAT- 2016/02/07 06:00
MHDA- 2017/05/10 06:00
PMCR- 2016/02/05
CRDT- 2016/02/07 06:00
PHST- 2015/11/05 00:00 [received]
PHST- 2016/02/03 00:00 [accepted]
PHST- 2016/02/07 06:00 [entrez]
PHST- 2016/02/07 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2016/02/05 00:00 [pmc-release]
AID - pp.15.01724 [pii]
AID - PP201501724R3 [pii]
AID - 10.1104/pp.15.01724 [doi]
PST - ppublish
SO  - Plant Physiol. 2016 Apr;170(4):2340-50. doi: 10.1104/pp.15.01724. Epub 2016 Feb 
      5.