PMID- 26850833
OWN - NLM
STAT- MEDLINE
DCOM- 20170724
LR  - 20231213
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 45
IP  - 10
DP  - 2016 Nov
TI  - THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral 
      squamous cell carcinoma.
PG  - 730-739
LID - 10.1111/jop.12430 [doi]
AB  - BACKGROUND: THBS1 (thrombospondin-1) is the extracellular matrix (ECM) protein 
      that affects diverse cellular activities. It constitutes the tumor stroma, but 
      the role of THBS1 in oral squamous cell carcinoma (OSCC) development is unclear. 
      The aim of this study was to clarify the relevance of THBS1 in the pathogenesis 
      of OSCC. MATERIALS AND METHODS: The expression of THBS1 was examined in 44 OSCC 
      by immunohistochemical analysis and in 43 OSCC by cDNA microarray analysis. Cell 
      culture experiments were conducted using human OSCC cell lines HSC3 and HO1N1 and 
      mouse fibroblast ST2 cells to examine the effect of TGFB1 on THBS1 expression, 
      and the effect of THBS1 on cellular behaviors. RESULTS: THBS1 was specifically 
      induced in the tumor microenvironment of OSCC. THBS1 appeared to be produced 
      mainly by the stromal cells, but also by OSCC cells. TGFB1 stimulated THBS1 
      expression in ST2, primary fibroblasts, and the OSCC cells. THBS1 promoted 
      migration and invasion of HSC3 and HO1N1 in transwell migration assays. THBS1 
      stimulated the expression of MMP3 (matrix metalloprotease 3), MMP9, MMP11, and 
      MMP13 in ST2 cells and MMP3, MMP11, and MMP13 in HO1N1 cells. The RGD peptide 
      suppressed the THBS1-stimulated migration and upregulation of MMP11 and MMP13. 
      CONCLUSIONS: THBS1 is a tumor-specific ECM protein that is induced by TGFB1 and 
      promotes migration of cancer cells and stimulates the expression of MMPs partly 
      through the integrin signaling, thereby favoring OSCC invasion.
CI  - (c) 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Pal, Samir Kumar
AU  - Pal SK
AD  - Department of Oral Pathology, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo, Japan.
AD  - Global Center of Excellence (GCOE) Program, International Research Center for 
      Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental 
      University, Tokyo, Japan.
FAU - Nguyen, Chi Thi Kim
AU  - Nguyen CT
AD  - Department of Oral Pathology, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo, Japan.
FAU - Morita, Kei-Ichi
AU  - Morita KI
AD  - Department of Oral Surgery, Graduate School of Medical and Dental Sciences, Tokyo 
      Medical and Dental University, Tokyo, Japan.
FAU - Miki, Yoshio
AU  - Miki Y
AD  - Global Center of Excellence (GCOE) Program, International Research Center for 
      Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental 
      University, Tokyo, Japan.
AD  - Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and 
      Dental University, Tokyo, Japan.
FAU - Kayamori, Kou
AU  - Kayamori K
AD  - Department of Oral Pathology, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo, Japan.
FAU - Yamaguchi, Akira
AU  - Yamaguchi A
AD  - Department of Oral Pathology, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo, Japan.
AD  - Global Center of Excellence (GCOE) Program, International Research Center for 
      Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental 
      University, Tokyo, Japan.
AD  - Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
FAU - Sakamoto, Kei
AU  - Sakamoto K
AD  - Department of Oral Pathology, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo, Japan. s-kei.mpa@tmd.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20160205
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (DNA, Complementary)
RN  - 0 (Thrombospondin 1)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (thrombospondin-1, human)
RN  - 0 (Thbs1 protein, mouse)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/enzymology/*metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - DNA, Complementary/metabolism
MH  - Head and Neck Neoplasms/enzymology/*metabolism/*pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Metalloendopeptidases/biosynthesis
MH  - Mice
MH  - Mouth Neoplasms/enzymology/*metabolism/*pathology
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Stromal Cells/enzymology/metabolism/pathology
MH  - Thrombospondin 1/*biosynthesis
MH  - Transforming Growth Factor beta1/*pharmacology
MH  - Up-Regulation
OTO - NOTNLM
OT  - TGFB1
OT  - THBS1
OT  - TSP-1
OT  - integrin
OT  - oral cancer
OT  - thrombospondin
EDAT- 2016/10/27 06:00
MHDA- 2017/07/25 06:00
CRDT- 2016/02/07 06:00
PHST- 2016/01/04 00:00 [accepted]
PHST- 2016/10/27 06:00 [pubmed]
PHST- 2017/07/25 06:00 [medline]
PHST- 2016/02/07 06:00 [entrez]
AID - 10.1111/jop.12430 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2016 Nov;45(10):730-739. doi: 10.1111/jop.12430. Epub 2016 Feb 
      5.