PMID- 26853742
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 500
DP  - 2016 May 1
TI  - Amperometric determination of serum total cholesterol with nanoparticles of 
      cholesterol esterase and cholesterol oxidase.
PG  - 6-11
LID - S0003-2697(16)00042-7 [pii]
LID - 10.1016/j.ab.2016.01.019 [doi]
AB  - We describe the preparation of glutaraldehyde cross-linked and functionalized 
      cholesterol esterase nanoparticles (ChENPs) and cholesterol oxidase nanoparticles 
      (ChOxNPs) aggregates and their co-immobilization onto Au electrode for improved 
      amperometric determination of serum total cholesterol. Transmission electron 
      microscope (TEM) images of ChENPs and ChOxNPs showed their spherical shape and 
      average size of 35.40 and 56.97 nm, respectively. Scanning electron microscope 
      (SEM) studies of Au electrode confirmed the co-immobilization of enzyme 
      nanoparticles (ENPs). The biosensor exhibited optimal response at pH 5.5 and 40 degrees C 
      within 5 s when polarized at +0.25 V versus Ag/AgCl. The working/linear range of 
      the biosensor was 10-700 mg/dl for cholesterol. The sensor showed high 
      sensitivity and measured total cholesterol as low as 0.1 mg/dl. The biosensor was 
      evaluated and employed for total cholesterol determination in sera of apparently 
      healthy and diseased persons. The analytical recovery of added cholesterol was 
      90%, whereas the within-batch and between-batch coefficients of variation (CVs) 
      were less than 2% and less than 3%. There was a good correlation (r = 0.99) 
      between serum cholesterol values as measured by the standard enzymic colorimetric 
      method and the current method. The initial activity of ENPs/working electrode was 
      reduced by 50% during its regular use (200 times) over a period of 60 days when 
      stored dry at 4 degrees C.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Aggarwal, V
AU  - Aggarwal V
AD  - Department of Biochemistry, Maharshi Dayanand (M.D.) University, Rohtak, Haryana, 
      124001, India.
FAU - Malik, J
AU  - Malik J
AD  - Centre for Biotechnology, Maharshi Dayanand (M.D.) University, Rohtak, Haryana, 
      124001, India.
FAU - Prashant, A
AU  - Prashant A
AD  - Centre for Biotechnology, Maharshi Dayanand (M.D.) University, Rohtak, Haryana, 
      124001, India.
FAU - Jaiwal, P K
AU  - Jaiwal PK
AD  - Centre for Biotechnology, Maharshi Dayanand (M.D.) University, Rohtak, Haryana, 
      124001, India.
FAU - Pundir, C S
AU  - Pundir CS
AD  - Department of Biochemistry, Maharshi Dayanand (M.D.) University, Rohtak, Haryana, 
      124001, India. Electronic address: pundircs@rediffmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160204
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.1.3.6 (Cholesterol Oxidase)
RN  - EC 3.1.1.13 (Sterol Esterase)
SB  - IM
MH  - Biosensing Techniques
MH  - Cholesterol/*blood
MH  - Cholesterol Oxidase/*metabolism
MH  - Electrochemical Techniques/*methods
MH  - Limit of Detection
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - *Nanoparticles
MH  - Sterol Esterase/*metabolism
OTO - NOTNLM
OT  - Bionanosensor
OT  - Cholesterol esterase nanoparticles
OT  - Cholesterol oxidase nanoparticles
OT  - Co-immobilization
OT  - Serum
OT  - Total cholesterol
EDAT- 2016/02/09 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/09 06:00
PHST- 2015/12/21 00:00 [received]
PHST- 2016/01/21 00:00 [revised]
PHST- 2016/01/21 00:00 [accepted]
PHST- 2016/02/09 06:00 [entrez]
PHST- 2016/02/09 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S0003-2697(16)00042-7 [pii]
AID - 10.1016/j.ab.2016.01.019 [doi]
PST - ppublish
SO  - Anal Biochem. 2016 May 1;500:6-11. doi: 10.1016/j.ab.2016.01.019. Epub 2016 Feb 
      4.