PMID- 26854180
OWN - NLM
STAT- MEDLINE
DCOM- 20160728
LR  - 20220316
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 148
DP  - 2016 Mar 1
TI  - Diclofenac pretreatment effects on the toll-like receptor 4/nuclear factor kappa 
      B-mediated inflammatory response to eccentric exercise in rat liver.
PG  - 247-53
LID - S0024-3205(16)30056-X [pii]
LID - 10.1016/j.lfs.2016.02.006 [doi]
AB  - Acute exercise is a stress stimulus that may cause cell damage through the 
      activation of the toll-like receptor (TLR)4 pathway, resulting in the 
      translocation of nuclear factor kappa B (NF-kappaB) into the cell nucleus and the 
      upregulation of inflammatory genes. Nonsteroidal anti-inflammatory drugs, such as 
      diclofenac, are often prescribed to counteract exercise-induced inflammation. 
      AIMS: This study analyzed effects of diclofenac pretreatment on the TLR4/NF-kappaB 
      pathway in rat liver after an acute eccentric exercise. MAIN METHODS: Twenty male 
      Wistar rats were divided in four groups: control-saline, control-diclofenac, 
      exercise-saline and exercise-diclofenac. The rats received saline or diclofenac 
      (10mg/kg) for 7days prior to an eccentric exercise bout. KEY FINDINGS: After 
      exercise there was an increase in TLR4, myeloid differentiation primary response 
      gene 88 (MyD88), TIR domain-containing adaptor inducing interferon (TRIF) and p65 
      NF-kappaB subunit protein levels. Exercise also resulted in increased mRNA and 
      protein expression of interleukin (IL)-6, inducible nitric oxide synthase (iNOS) 
      and tumor necrosis factor (TNF)-alpha. Proinflammatory effects of exercise were 
      prevented by the administration of diclofenac, which blunted the activation of 
      the TLR4/NF-kappaB pathway and the inflammatory response in the liver of exercised 
      rats. SIGNIFICANCE: Results from the present study highlight the role of TLR4 as 
      a target for anti-inflammatory interventions.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Barcelos, Romulo Pillon
AU  - Barcelos RP
AD  - Departamento de Bioquimica e Biologia Molecular, Centro de Ciencias Naturais e 
      Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 
      Brazil; Institute of Biomedicine (IBIOMED) and CIBERehd, University of Leon, 
      Campus Universitario, 24071 Leon, Spain. Electronic address: 
      romulo1604@hotmail.com.
FAU - Bresciani, Guilherme
AU  - Bresciani G
AD  - Instituto de Actividad Fisica y Salud, Universidad Autonoma de Chile, Chile.
FAU - Rodriguez-Miguelez, Paula
AU  - Rodriguez-Miguelez P
AD  - Division of Clinical Translational Science, Georgia Prevention Institute, 
      Department of Pediatrics, Georgia Regents University, USA.
FAU - Cuevas, Maria Jose
AU  - Cuevas MJ
AD  - Institute of Biomedicine (IBIOMED) and CIBERehd, University of Leon, Campus 
      Universitario, 24071 Leon, Spain.
FAU - Soares, Felix Alexandre Antunes
AU  - Soares FA
AD  - Departamento de Bioquimica e Biologia Molecular, Centro de Ciencias Naturais e 
      Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 
      Brazil.
FAU - Barbosa, Nilda Vargas
AU  - Barbosa NV
AD  - Departamento de Bioquimica e Biologia Molecular, Centro de Ciencias Naturais e 
      Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 
      Brazil.
FAU - Gonzalez-Gallego, Javier
AU  - Gonzalez-Gallego J
AD  - Institute of Biomedicine (IBIOMED) and CIBERehd, University of Leon, Campus 
      Universitario, 24071 Leon, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160204
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 144O8QL0L1 (Diclofenac)
SB  - IM
MH  - Animals
MH  - Diclofenac/*administration & dosage
MH  - Inflammation Mediators/antagonists & inhibitors/*metabolism
MH  - Liver/drug effects/*metabolism
MH  - Male
MH  - NF-kappa B/antagonists & inhibitors/*metabolism
MH  - Physical Conditioning, Animal/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Toll-Like Receptor 4/antagonists & inhibitors/*metabolism
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Acute exercise
OT  - Diclofenac
OT  - Inflammation
OT  - Liver
OT  - NF-kappaB
OT  - TLR4
EDAT- 2016/02/09 06:00
MHDA- 2016/07/29 06:00
CRDT- 2016/02/09 06:00
PHST- 2015/09/08 00:00 [received]
PHST- 2015/10/04 00:00 [revised]
PHST- 2016/02/03 00:00 [accepted]
PHST- 2016/02/09 06:00 [entrez]
PHST- 2016/02/09 06:00 [pubmed]
PHST- 2016/07/29 06:00 [medline]
AID - S0024-3205(16)30056-X [pii]
AID - 10.1016/j.lfs.2016.02.006 [doi]
PST - ppublish
SO  - Life Sci. 2016 Mar 1;148:247-53. doi: 10.1016/j.lfs.2016.02.006. Epub 2016 Feb 4.