PMID- 26854347
OWN - NLM
STAT- MEDLINE
DCOM- 20161011
LR  - 20191210
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 184
DP  - 2016 Feb 29
TI  - The viral non-structural protein 1 alpha (Nsp1alpha) inhibits p53 apoptosis activity 
      by increasing murine double minute 2 (mdm2) expression in porcine reproductive 
      and respiratory syndrome virus (PRRSV) early-infected cells.
PG  - 73-9
LID - S0378-1135(16)30009-8 [pii]
LID - 10.1016/j.vetmic.2016.01.009 [doi]
AB  - Apoptosis is one of the most important mechanisms of pathogenesis in porcine 
      reproductive and respiratory syndrome virus (PRRSV)-infected cells. The tumor 
      suppressor p53 plays a critical role in apoptotic induction in viral infections. 
      In the present study, we found that p53 activity was inhibited at the early stage 
      of PRRSV infection in both the highly pathogenic (HP) and lowly pathogenic (LP) 
      PRRSV isolates. Bax expression showed a similar change pattern to that of p53. 
      Murine double minute 2 (mdm2) expressed higher in PRRSV-infected cells than in 
      uninfected cells at the early stage of infection and promoted p53 degradation. We 
      show for the first time that the non-structural protein 1 alpha (Nsp1alpha) of PRRSV 
      is a negative regulator of p53 activity through increasing mdm2 expression and 
      p53 ubiquitination, while p53 is inhibitory to PRRSV replication at the early 
      stage of infection. We conclude that PRRSV manipulates the host factors mdm2 and 
      p53 via its Nsp1alpha for increased replication at the early stage of infection. 
      These provide a novel perspective to understand the interaction between apoptosis 
      and replication of PRRSV.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Wang, Xiaodu
AU  - Wang X
AD  - College of Animal Science and Technology, Zhejiang A&F University, Lin'an, China. 
      Electronic address: xiaoduwang@163.com.
FAU - Shao, Chunyan
AU  - Shao C
AD  - College of Animal Science and Technology, Zhejiang A&F University, Lin'an, China. 
      Electronic address: cyshao0101@163.com.
FAU - Wang, Luyan
AU  - Wang L
AD  - College of Animal Science and Technology, Zhejiang A&F University, Lin'an, China. 
      Electronic address: luyanhankesi@sina.com.
FAU - Li, Qunjing
AU  - Li Q
AD  - College of Animal Science and Technology, Zhejiang A&F University, Lin'an, China. 
      Electronic address: 646089770@qq.com.
FAU - Song, Houhui
AU  - Song H
AD  - College of Animal Science and Technology, Zhejiang A&F University, Lin'an, China. 
      Electronic address: songhh@zafu.edu.cn.
FAU - Fang, Weihuan
AU  - Fang W
AD  - College of Animal Science and Technology, Zhejiang A&F University, Lin'an, China; 
      Zhejiang University Institute of Preventive Veterinary Medicine, Zhejiang 
      Province Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China. 
      Electronic address: whfang@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160114
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chlorocebus aethiops
MH  - *Gene Expression Regulation
MH  - Porcine Reproductive and Respiratory Syndrome/*physiopathology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Proto-Oncogene Proteins c-mdm2/*genetics
MH  - Swine
MH  - Tumor Suppressor Protein p53/*metabolism
MH  - Ubiquitination
MH  - Viral Nonstructural Proteins/*metabolism
MH  - Virus Replication/physiology
MH  - bcl-2-Associated X Protein/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Porcine reproductive and respiratory syndrome virus
OT  - mdm2
OT  - p53
EDAT- 2016/02/09 06:00
MHDA- 2016/10/12 06:00
CRDT- 2016/02/09 06:00
PHST- 2015/08/13 00:00 [received]
PHST- 2016/01/09 00:00 [revised]
PHST- 2016/01/11 00:00 [accepted]
PHST- 2016/02/09 06:00 [entrez]
PHST- 2016/02/09 06:00 [pubmed]
PHST- 2016/10/12 06:00 [medline]
AID - S0378-1135(16)30009-8 [pii]
AID - 10.1016/j.vetmic.2016.01.009 [doi]
PST - ppublish
SO  - Vet Microbiol. 2016 Feb 29;184:73-9. doi: 10.1016/j.vetmic.2016.01.009. Epub 2016 
      Jan 14.