PMID- 26854444
OWN - NLM
STAT- MEDLINE
DCOM- 20160314
LR  - 20210909
IS  - 1107-0625 (Print)
IS  - 1107-0625 (Linking)
VI  - 20
IP  - 6
DP  - 2015 Nov-Dec
TI  - Inhibitory effect of Aphidicolin - a tetracyclic diterpene - on the proliferation 
      and apoptotic induction in human cervical cancer (HeLa) cells.
PG  - 1480-6
AB  - PURPOSE: The objective of the present work was to investigate the antitumor and 
      apoptotic effect of aphidicolin against human cervical cancer (HeLa) cells. 
      METHODS: Flow cytometry was performed to detect alterations in the mitochondrial 
      membrane potential loss (DeltaPsim) after aphidicolin treatment. Cell viability was 
      assessed by the MTT assay while the apoptotic effects of the compound were 
      examined by fluorescence microscopy and flow cytometry. RESULTS: Aphidicolin 
      induced dose-dependent as well as time-dependent cytotoxic effects in HeLa cells. 
      Chromatin condensation and formation of apoptotic bodies were observed by Hoechst 
      33258 staining after drug treatment. Aphidicolin induced both early and late 
      apoptosis in HeLa cells which were correlated with strong concentration of the 
      compound. DeltaPsim was also observed following aphidicolin treatment at varying doses. 
      CONCLUSION: Aphidicolin is a potent antitumor and apoptotic agent against human 
      cervical carcinoma and its effects are mediated via chromatin condensation and 
      mitochondrial membrane potential loss.
FAU - Yu, En-Yan
AU  - Yu EY
AD  - Department of Obstetrics and Gynecology, Shandong University Hospital, Jinan 
      250100, China.
FAU - Zhao, Rui-Yan
AU  - Zhao RY
FAU - Wang, Dong-Sheng
AU  - Wang DS
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 38966-21-1 (Aphidicolin)
SB  - IM
RIN - J BUON. 2021 Jan-Feb;26(1):291. PMID: 33721476
MH  - Aphidicolin/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Uterine Cervical Neoplasms/*drug therapy/pathology
EDAT- 2016/02/09 06:00
MHDA- 2016/03/15 06:00
CRDT- 2016/02/09 06:00
PHST- 2021/02/28 00:00 [retracted]
PHST- 2016/02/09 06:00 [entrez]
PHST- 2016/02/09 06:00 [pubmed]
PHST- 2016/03/15 06:00 [medline]
PST - ppublish
SO  - J BUON. 2015 Nov-Dec;20(6):1480-6.