PMID- 26856331
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 52
IP  - 2
DP  - 2016 Apr
TI  - Immunopotentiation of four natural adjuvants co-administered with a highly 
      pathogenic porcine reproductive and respiratory syndrome virus glycoprotein 5 
      subunit.
PG  - 261-9
LID - 10.1007/s11262-016-1299-9 [doi]
AB  - To improve the efficacy of porcine reproductive and respiratory syndrome virus 
      (PRRSV) subunit vaccine, the immunological enhancement effects of four natural 
      adjuvants were compared, including Astragalus extract, Astragalus/Bacillus 
      production, propolis, and Freund's adjuvant. The results showed that titers of 
      IgG and neutralizing antibody against highly pathogenic PRRSV (HP-PRRSV) 
      glycoprotein 5 (GP5) from the groups of Astragalus/Bacillus adjuvant and Freund's 
      adjuvant co-administered with a recombinant HP-PRRSV GP5 subunit were the highest 
      among all the experimental groups. In cellular immunity, as shown in T lymphocyte 
      proliferation level, Astragalus/Bacillus adjuvant and Freund's adjuvant exhibited 
      the most potent immunological enhancement effects. Exceptionally, pigs inoculated 
      with Freund's adjuvant developed severely delayed humoral immune responses within 
      28 days post inoculation. The adjuvants of Astragalus extract and propolis also 
      exhibited immunological enhancement effects on humoral immune and cell-mediated 
      immune responses, but which were obviously lower than Astragalus/Bacillus 
      adjuvant and Freund's adjuvant. Following challenge with HP-PRRSV, pigs 
      inoculated with Astragalus/Bacillus adjuvant and Freund's adjuvant 
      co-administered with GP5 subunit showed lower viremia, slighter clinical signs, 
      and less pathological lung lesions compared with the groups inoculated using 
      Astragalus extract and propolis. In conclusion, from immune responses and 
      immunological protection against HP-PRRSV, the Astragalus/Bacillus adjuvant 
      demonstrated the most potent immunological enhancement effect on HP-PRRSV GP5 
      subunit. Astragalus/Bacillus adjuvant showed promise as a candidate immune 
      adjuvant, to more efficiently prevent and control PRRS.
FAU - Peng, Jun
AU  - Peng J
AD  - College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Taian, China.
FAU - Yuan, Yanmei
AU  - Yuan Y
AD  - College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Taian, China.
FAU - Shen, Si
AU  - Shen S
AD  - College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Taian, China.
FAU - Niu, Zhongxiang
AU  - Niu Z
AD  - College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Taian, China.
FAU - Du, Yijun
AU  - Du Y
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Sangyuan Road No. 10, Jinan, 250100, China.
FAU - Wu, Jiaqiang
AU  - Wu J
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Sangyuan Road No. 10, Jinan, 250100, China.
FAU - Li, Jun
AU  - Li J
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Sangyuan Road No. 10, Jinan, 250100, China.
FAU - Yu, Jiang
AU  - Yu J
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Sangyuan Road No. 10, Jinan, 250100, China.
FAU - Wang, Tao
AU  - Wang T
AD  - College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Taian, China.
FAU - Wang, Jinbao
AU  - Wang J
AD  - College of Veterinary Medicine, Shandong Agricultural University, Taian, China. 
      wangjb@saas.ac.cn.
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Sangyuan Road No. 10, Jinan, 250100, China. 
      wangjb@saas.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160208
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, Subunit)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antibodies, Viral
MH  - Immunoglobulin G/blood/immunology
MH  - Lymphocyte Activation/immunology
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Recombinant Fusion Proteins
MH  - Swine
MH  - Vaccines, Subunit/*administration & dosage/*immunology
MH  - Viral Envelope Proteins/*immunology
MH  - Viral Load
OTO - NOTNLM
OT  - Immunological enhancement effect
OT  - Natural immune adjuvant
OT  - Porcine reproductive and respiratory syndrome virus subunit
EDAT- 2016/02/10 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/10 06:00
PHST- 2015/11/16 00:00 [received]
PHST- 2016/01/23 00:00 [accepted]
PHST- 2016/02/10 06:00 [entrez]
PHST- 2016/02/10 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s11262-016-1299-9 [pii]
AID - 10.1007/s11262-016-1299-9 [doi]
PST - ppublish
SO  - Virus Genes. 2016 Apr;52(2):261-9. doi: 10.1007/s11262-016-1299-9. Epub 2016 Feb 
      8.