PMID- 26856458
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1532-8511 (Electronic)
IS  - 1052-3057 (Linking)
VI  - 25
IP  - 4
DP  - 2016 Apr
TI  - Aneurysm Organization Effects of Gellan Sulfate Core Platinum Coil with 
      Tenascin-C in a Simulated Clinical Setting and the Possible Mechanism.
PG  - 771-80
LID - S1052-3057(15)00677-1 [pii]
LID - 10.1016/j.jstrokecerebrovasdis.2015.12.010 [doi]
AB  - BACKGROUND: This study aimed to deliver gellan sulfate core platinum coil with 
      tenascin-C (GSCC-TNC) into rabbit side-wall aneurysms endovascularly and to 
      evaluate the organization effects in a simulated clinical setting. METHODS: 
      Elastase-induced rabbit side-wall aneurysms were randomly coiled via a 
      transfemoral route like clinical settings with platinum coils (PCs), gellan 
      sulfate core platinum coils (GSCCs), or GSCC-TNCs (n = 5, respectively). 
      Aneurysm-occlusion status was evaluated angiographically and histologically at 2 
      weeks post coiling. As each rabbit coiled aneurysm provided only 2-3 tissue 
      slices due to technical limitations and prevented immunohistochemical 
      evaluations, a PC, GSCC, or GSCC-TNC was randomly implanted in a rat blind-ended 
      model (n = 3, respectively) and the organization effects were 
      immunohistochemically evaluated for expressions of tenascin-C (TNC), transforming 
      growth factor-beta (TGF-beta), and matrix metalloproteinase-9 (MMP-9) 2 weeks later. 
      RESULTS: Coil handling was similar among the 3 kinds of coils. GSCCs showed a 
      significantly higher ratio of organized area to the aneurysmal cavity than PCs, 
      but GSCC-TNCs had the greatest organization-promoting effects on aneurysms (the 
      ratio of organized area/aneurysmal luminal area: PC, 17.9 +/- 7.1%; GSCC, 
      54.2 +/- 18.3%; GSCC-TNC, 82.5 +/- 5.8%). GSCC-TNCs had intense immunoreactivities 
      for TNC, TGF-beta, and MMP-9 in the organized thrombosis and tunica media. GSCCs 
      also showed intense immunoreactivities for TNC, TGF-beta, and MMP-9, although the 
      extent was less than GSCC-TNCs. The immunoreactivities were hardly found in 
      unorganized thrombus and the tunica media of aneurysm wall in the PC group. 
      CONCLUSIONS: This study first showed that GSCC-TNCs promote intra-aneurysmal clot 
      organization in simulated clinical settings using rabbits possibly through the 
      TGF-beta and MMP-9 upregulation.
CI  - Copyright (c) 2016 National Stroke Association. Published by Elsevier Inc. All 
      rights reserved.
FAU - Miura, Yoichi
AU  - Miura Y
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan. Electronic address: yoimiu@hotmail.com.
FAU - Tanemura, Hiroshi
AU  - Tanemura H
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
FAU - Fujimoto, Masashi
AU  - Fujimoto M
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
FAU - Hamada, Kazuhide
AU  - Hamada K
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
FAU - Miyamoto, Keiichi
AU  - Miyamoto K
AD  - Division of Chemistry for Materials, Faculty of Engineering and Graduate School 
      of Engineering, Mie University, Tsu, Japan.
FAU - Toma, Naoki
AU  - Toma N
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
FAU - Imanaka-Yoshida, Kyoko
AU  - Imanaka-Yoshida K
AD  - Departments of Pathology and Matrix Biology, Mie University Graduate School of 
      Medicine, Tsu, Japan.
FAU - Matsushima, Satoshi
AU  - Matsushima S
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
FAU - Yoshida, Toshimichi
AU  - Yoshida T
AD  - Departments of Pathology and Matrix Biology, Mie University Graduate School of 
      Medicine, Tsu, Japan.
FAU - Taki, Waro
AU  - Taki W
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
FAU - Suzuki, Hidenori
AU  - Suzuki H
AD  - Department of Neurosurgery, Mie University Graduate School of Medicine, Tsu, 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160205
PL  - United States
TA  - J Stroke Cerebrovasc Dis
JT  - Journal of stroke and cerebrovascular diseases : the official journal of National 
      Stroke Association
JID - 9111633
RN  - 0 (Polysaccharides)
RN  - 0 (Sulfuric Acid Esters)
RN  - 0 (Tenascin)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (gellan sulfate)
RN  - 49DFR088MY (Platinum)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Angiography
MH  - Animals
MH  - Cell Line, Tumor
MH  - Disease Models, Animal
MH  - Embolization, Therapeutic/*methods
MH  - Female
MH  - Glioma/pathology
MH  - Intracranial Aneurysm/*pathology/*therapy
MH  - Male
MH  - Matrix Metalloproteinase 9/metabolism
MH  - *Platinum
MH  - Polysaccharides/*therapeutic use
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sulfuric Acid Esters/*therapeutic use
MH  - Tenascin/*metabolism
MH  - Transforming Growth Factor beta/metabolism
OTO - NOTNLM
OT  - Tenascin-C
OT  - coil embolization
OT  - elastase-induced rabbit aneurysms
OT  - gellan sulfate
OT  - transforming growth factor-beta
EDAT- 2016/02/10 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/10 06:00
PHST- 2015/07/21 00:00 [received]
PHST- 2015/11/11 00:00 [revised]
PHST- 2015/12/10 00:00 [accepted]
PHST- 2016/02/10 06:00 [entrez]
PHST- 2016/02/10 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S1052-3057(15)00677-1 [pii]
AID - 10.1016/j.jstrokecerebrovasdis.2015.12.010 [doi]
PST - ppublish
SO  - J Stroke Cerebrovasc Dis. 2016 Apr;25(4):771-80. doi: 
      10.1016/j.jstrokecerebrovasdis.2015.12.010. Epub 2016 Feb 5.