PMID- 26858596
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160209
LR  - 20200930
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 9
DP  - 2015
TI  - Electrophysiological Evidence for a Direct Link between the Main and Accessory 
      Olfactory Bulbs in the Adult Rat.
PG  - 518
LID - 10.3389/fnins.2015.00518 [doi]
LID - 518
AB  - It is accepted that the main- and accessory- olfactory systems exhibit 
      overlapping responses to pheromones and odorants. We performed whole-cell 
      patch-clamp recordings in adult rat olfactory bulb slices to define a possible 
      interaction between the first central relay of these systems: the accessory 
      olfactory bulb (AOB) and the main olfactory bulb (MOB). This was tested by 
      applying electrical field stimulation in the dorsal part of the MOB while 
      recording large principal cells (LPCs) of the anterior AOB (aAOB). Additional 
      recordings of LPCs were performed at either side of the plane of intersection 
      between the aAOB and posterior-AOB (pAOB) halves, or linea alba, while applying 
      field stimulation to the opposite half. A total of 92 recorded neurons were 
      filled during whole-cell recordings with biocytin and studied at the light 
      microscope. Neurons located in the aAOB (n = 6, 8%) send axon collaterals to the 
      MOB since they were antidromically activated in the presence of glutamate 
      receptor antagonists (APV and CNQX). Recorded LPCs evoked orthodromic excitatory 
      post-synaptic responses (n = 6, aAOB; n = 1, pAOB) or antidromic action 
      potentials (n = 8, aAOB; n = 7, pAOB) when applying field stimulation to the 
      opposite half of the recording site (e.g., recording in aAOB; stimulating in 
      pAOB, and vice-versa). Observation of the filled neurons revealed that indeed, 
      LPCs send axon branches that cross the linea alba to resolve in the internal 
      cellular layer. Additionally, LPCs of the aAOB send axon collaterals to 
      dorsal-MOB territory. Notably, while performing AOB recordings we found a 
      sub-population of neurons (24% of the total) that exhibited voltage-dependent 
      bursts of action potentials. Our findings support the existence of: 1. a direct 
      projection from aAOB LPCs to dorsal-MOB, 2. physiologically active synapses 
      linking aAOB and pAOB, and 3. pacemaker-like neurons in both AOB halves. This 
      work was presented in the form of an Abstract on SfN 2014 (719.14/EE17).
FAU - Vargas-Barroso, Victor
AU  - Vargas-Barroso V
AD  - Neurobiologia del Desarrollo y Neurofisiologia, Instituto de Neurobiologia, 
      Universidad Nacional Autonoma de Mexico, Campus Juriquilla Queretaro, Mexico.
FAU - Ordaz-Sanchez, Benito
AU  - Ordaz-Sanchez B
AD  - Neurobiologia del Desarrollo y Neurofisiologia, Instituto de Neurobiologia, 
      Universidad Nacional Autonoma de Mexico, Campus Juriquilla Queretaro, Mexico.
FAU - Pena-Ortega, Fernando
AU  - Pena-Ortega F
AD  - Neurobiologia del Desarrollo y Neurofisiologia, Instituto de Neurobiologia, 
      Universidad Nacional Autonoma de Mexico, Campus Juriquilla Queretaro, Mexico.
FAU - Larriva-Sahd, Jorge A
AU  - Larriva-Sahd JA
AD  - Neurobiologia del Desarrollo y Neurofisiologia, Instituto de Neurobiologia, 
      Universidad Nacional Autonoma de Mexico, Campus Juriquilla Queretaro, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20160126
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC4726767
OTO - NOTNLM
OT  - accessory olfactory bulb
OT  - bursting
OT  - main olfactory bulb
OT  - patch-clamp
EDAT- 2016/02/10 06:00
MHDA- 2016/02/10 06:01
PMCR- 2015/01/01
CRDT- 2016/02/10 06:00
PHST- 2015/07/02 00:00 [received]
PHST- 2015/12/22 00:00 [accepted]
PHST- 2016/02/10 06:00 [entrez]
PHST- 2016/02/10 06:00 [pubmed]
PHST- 2016/02/10 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2015.00518 [doi]
PST - epublish
SO  - Front Neurosci. 2016 Jan 26;9:518. doi: 10.3389/fnins.2015.00518. eCollection 
      2015.