PMID- 26858641
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160209
LR  - 20200930
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 6
DP  - 2015
TI  - NADPH Oxidase/ROS-Dependent VCAM-1 Induction on TNF-alpha-Challenged Human Cardiac 
      Fibroblasts Enhances Monocyte Adhesion.
PG  - 310
LID - 10.3389/fphar.2015.00310 [doi]
LID - 310
AB  - The inflammation-dependent adhesion molecule expressions are characterized in 
      cardiovascular diseases and myocardial tissue infiltrations. Several 
      pro-inflammatory cytokines are elevated in the acute myocardial injury and 
      infarction. Tumor necrosis factor-alpha (TNF-alpha), a pro-inflammatory cytokine, is 
      raised in the injury tissues and inflammatory regions and involved in the 
      pathogenesis of cardiac injury, inflammation, and apoptosis. In fibroblasts, 
      TNF-alpha-triggered expression of vascular cell adhesion molecule (VCAM)-1 aggravated 
      the heart inflammation. However, the mechanisms underlying TNF-alpha-mediated VCAM-1 
      expression in cardiac fibroblasts remain unclear. Here, the primary cultured 
      human cardiac fibroblasts (HCFs) were used to investigate the effects of TNF-alpha on 
      VCAM-1 expression. The molecular evidence, including protein, mRNA, and promoter 
      analyses, indicated that TNF-alpha-induced VCAM-1 gene expression is mediated through 
      the TNFR-dependent manner. Activation of TNF-alpha/TNFR system triggered 
      PKCalpha-dependent NADPH oxidase (Nox)/reactive oxygen species (ROS) signal linking 
      to MAPK cascades, and then led to activation of the transcription factor, AP-1. 
      Moreover, the results of mRNA and promoter assay demonstrated that c-Jun/AP-1 
      phosphorylated by TNF-alpha turns on VCAM-1 gene expression. Subsequently, 
      up-regulated VCAM-1 on the cell surface of TNF-alpha-challenged HCFs increased the 
      number of monocytes adhering to these cells. These results indicated that in 
      HCFs, activation of AP-1 by PKCalpha-dependent Nox/ROS/MAPKs cascades is required for 
      TNF-alpha-induced VCAM-1 expression. To clarify the mechanisms of TNF-alpha-induced 
      VCAM-1 expression in HCFs may provide therapeutic strategies for heart injury and 
      inflammatory diseases.
FAU - Lin, Chih-Chung
AU  - Lin CC
AD  - Department of Anesthetics, Chang Gung Memorial Hospital at Linkou, and College of 
      Medicine, Chang Gung University Tao-Yuan, Taiwan.
FAU - Yang, Chien-Chung
AU  - Yang CC
AD  - Department of Physiology and Pharmacology and Health Aging Research Center, 
      College of Medicine, Chang Gung UniversityTao-Yuan, Taiwan; Department of 
      Traditional Chinese Medicine, Chang Gung Memorial Hospital at Lin-KouTao-Yuan, 
      Taiwan.
FAU - Wang, Chen-Yu
AU  - Wang CY
AD  - Department of Anesthetics, Chang Gung Memorial Hospital at Linkou, and College of 
      Medicine, Chang Gung University Tao-Yuan, Taiwan.
FAU - Tseng, Hui-Ching
AU  - Tseng HC
AD  - Department of Physiology and Pharmacology and Health Aging Research Center, 
      College of Medicine, Chang Gung University Tao-Yuan, Taiwan.
FAU - Pan, Chih-Shuo
AU  - Pan CS
AD  - Department of Physiology and Pharmacology and Health Aging Research Center, 
      College of Medicine, Chang Gung University Tao-Yuan, Taiwan.
FAU - Hsiao, Li-Der
AU  - Hsiao LD
AD  - Department of Anesthetics, Chang Gung Memorial Hospital at Linkou, and College of 
      Medicine, Chang Gung University Tao-Yuan, Taiwan.
FAU - Yang, Chuen-Mao
AU  - Yang CM
AD  - Department of Physiology and Pharmacology and Health Aging Research Center, 
      College of Medicine, Chang Gung UniversityTao-Yuan, Taiwan; Research Center for 
      Industry of Human Ecology and Graduate Institute of Health Industry Technology, 
      Chang Gung University of Science and TechnologyTao-Yuan, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20160128
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC4729888
OTO - NOTNLM
OT  - NADPH oxidase
OT  - ROS
OT  - TNF-alpha
OT  - VCAM-1
OT  - cardiac fibroblasts
OT  - transcription factor
EDAT- 2016/02/10 06:00
MHDA- 2016/02/10 06:01
PMCR- 2016/01/28
CRDT- 2016/02/10 06:00
PHST- 2015/09/07 00:00 [received]
PHST- 2015/12/16 00:00 [accepted]
PHST- 2016/02/10 06:00 [entrez]
PHST- 2016/02/10 06:00 [pubmed]
PHST- 2016/02/10 06:01 [medline]
PHST- 2016/01/28 00:00 [pmc-release]
AID - 10.3389/fphar.2015.00310 [doi]
PST - epublish
SO  - Front Pharmacol. 2016 Jan 28;6:310. doi: 10.3389/fphar.2015.00310. eCollection 
      2015.