PMID- 26862668
OWN - NLM
STAT- MEDLINE
DCOM- 20161214
LR  - 20161230
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 26
IP  - 1
DP  - 2016
TI  - Activation of transcription factors in human bronchial epithelial cells exposed 
      to aqueous extracts of mainstream cigarette smoke in vitro.
PG  - 22-31
LID - 10.3109/15376516.2015.1123788 [doi]
AB  - This study aimed to identify the most sensitive transcription factor activated by 
      cigarette smoke extract (CSE) and to explore cigarette smoke components that have 
      high biological activities in a cell-base assay. Previously, we found evidence 
      that implicated 10 different transcription factors as having a high biological 
      activity to CSE in vitro, based on the results of a comprehensive gene expression 
      profile. For this study, luciferase reporter assays for each transcription factor 
      were developed in two types of human bronchial epithelial cells: NCI-H292 and 
      BEAS-2B cells. The results demonstrated that the nuclear factor erythroid 
      2-related factor 2 (NRF2)/anti-oxidant response element (ARE) pathway was the 
      most sensitive in response to CSE. Consistently, hemo oxygenase-1 (HO-1), a 
      downstream target gene of NRF2, was effectively up-regulated in BEAS-2B cells 
      exposed to CSE. Moreover, among 1395 cigarette smoke components, naphthoquinones 
      including 9,10-phenaotrenquinone, quinones, benzenediols and alpha, beta-unsaturated 
      carbonyls, were identified as major smoke components that contribute to 
      activating the NRF2/ARE pathway, as indicated by the ARE-reporter assay in 
      BEAS-2B cells. Taken together, NRF2 appears to be a key molecule in the 
      CSE-induced cellular response, and the employed methodology is helpful for the 
      analysis of molecular and cellular effects by CSE.
FAU - Sekine, Takashi
AU  - Sekine T
AD  - a R&D Group, Tobacco Business Headquarters, Scientific Product Assessment Center, 
      Japan Tobacco Inc. , Yohohama , Japan.
FAU - Hirata, Tadashi
AU  - Hirata T
AD  - a R&D Group, Tobacco Business Headquarters, Scientific Product Assessment Center, 
      Japan Tobacco Inc. , Yohohama , Japan.
FAU - Mine, Toshiki
AU  - Mine T
AD  - a R&D Group, Tobacco Business Headquarters, Scientific Product Assessment Center, 
      Japan Tobacco Inc. , Yohohama , Japan.
FAU - Fukano, Yasuo
AU  - Fukano Y
AD  - a R&D Group, Tobacco Business Headquarters, Scientific Product Assessment Center, 
      Japan Tobacco Inc. , Yohohama , Japan.
LA  - eng
PT  - Journal Article
DEP - 20160210
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (Complex Mixtures)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Smoke)
RN  - 0 (Transcription Factors)
RN  - 059QF0KO0R (Water)
RN  - 261QK3SSBH (Neutral Red)
SB  - IM
MH  - Bronchi/cytology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Complex Mixtures/chemistry/*toxicity
MH  - Epithelial Cells/*drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Neutral Red
MH  - Reactive Oxygen Species
MH  - Smoke/*adverse effects/analysis
MH  - *Tobacco Products
MH  - Transcription Factors/genetics/*metabolism
MH  - Water/chemistry
OTO - NOTNLM
OT  - 9,10-phenanthraquinone
OT  - BEAS-2B
OT  - NCI-H292
OT  - anti-oxidant response element
OT  - nuclear factor erythroid 2-related factor 2
EDAT- 2016/02/11 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/11 06:00
PHST- 2016/02/11 06:00 [entrez]
PHST- 2016/02/11 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.3109/15376516.2015.1123788 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2016;26(1):22-31. doi: 10.3109/15376516.2015.1123788. Epub 
      2016 Feb 10.