PMID- 26865236
OWN - NLM
STAT- MEDLINE
DCOM- 20160505
LR  - 20160211
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 118
IP  - 1
DP  - 2016 Feb 11
TI  - Identification and characterization of ostreid herpesvirus 1 associated with 
      massive mortalities of Scapharca broughtonii broodstocks in China.
PG  - 65-75
LID - 10.3354/dao02958 [doi]
AB  - In the early summer of 2012 and 2013, mass mortalities of blood ark shell 
      (Scapharca [Anadara] broughtonii), broodstocks were reported in several 
      hatcheries on the coast of northern China. Clinical signs including slow 
      response, gaping valves and pale visceral mass were observed in diseased 
      individuals. In response to these reported mortalities, 238 samples were 
      collected from hatcheries at 6 sites. Microscopic changes including lysed 
      connective tissue, dilation of the digestive tubules, eosinophilic inclusion 
      bodies, nuclear chromatin margination and pyknosis were found in affected 
      animals. Transmission electron microscopy (TEM) revealed herpes-like viral 
      particles within the connective tissue of the mantle. Quantative PCR (qPCR) and 
      nested PCR (nPCR) analysis using primers specific for ostreid herpesvirus 1 
      (OsHV-1) indicated significant higher prevalence of OsHV-1 DNA in cases 
      associated with mass mortalities than those without mass mortalities (p = 0.0012 
      for qPCR, p < 0.0001 for nPCR). qPCR also indicated that samples associated with 
      mass mortalities carried high viral DNA loads, while the loads in apparently 
      healthy samples were significantly lower (t = 3.15, df = 92, p = 0.002). Sequence 
      analysis of the C2/C6 region of nPCR products revealed 5 newly described 
      variants, which were closely related to each other. Phylogenetic analysis of the 
      5 virus variants and 48 virus variants reported in previous studies identified 2 
      main phylogenetic groups, and the 5 virus variants identified here were allocated 
      to a separate subclade. To our knowledge, this is the first report of mass 
      mortalities of bivalve broodstocks associated with OsHV-1 infection.
FAU - Bai, Changming
AU  - Bai C
AD  - Division of Maricultural Organism Disease Control and Molecular Pathology, Yellow 
      Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 
      Nanjing Road, Qingdao 266071, PR China.
FAU - Gao, Wenhui
AU  - Gao W
FAU - Wang, Chongming
AU  - Wang C
FAU - Yu, Tao
AU  - Yu T
FAU - Zhang, Tianwen
AU  - Zhang T
FAU - Qiu, Zhaoxing
AU  - Qiu Z
FAU - Wang, Qingchen
AU  - Wang Q
FAU - Huang, Jie
AU  - Huang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Bivalvia/*virology
MH  - China
MH  - Genetic Variation
MH  - Herpesviridae/genetics/isolation & purification/*physiology
MH  - Host-Pathogen Interactions
MH  - Phylogeny
EDAT- 2016/02/13 06:00
MHDA- 2016/05/06 06:00
CRDT- 2016/02/12 06:00
PHST- 2016/02/12 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2016/05/06 06:00 [medline]
AID - 10.3354/dao02958 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2016 Feb 11;118(1):65-75. doi: 10.3354/dao02958.