PMID- 26865698
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20220408
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 6
IP  - 4
DP  - 2016 Apr 7
TI  - Fluorescence In Situ Hybridization (FISH)-Based Karyotyping Reveals Rapid 
      Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus 
      vulgaris) and Relatives.
PG  - 1013-22
LID - 10.1534/g3.115.024984 [doi]
AB  - Fluorescence in situ hybridization (FISH)-based karyotyping is a powerful 
      cytogenetics tool to study chromosome organization, behavior, and chromosome 
      evolution. Here, we developed a FISH-based karyotyping system using a probe 
      mixture comprised of centromeric and subtelomeric satellite repeats, 5S rDNA, and 
      chromosome-specific BAC clones in common bean, which enables one to unambiguously 
      distinguish all 11 chromosome pairs. Furthermore, we applied the karyotyping 
      system to several wild relatives and landraces of common bean from two distinct 
      gene pools, as well as other related Phaseolus species, to investigate repeat 
      evolution in the genus Phaseolus Comparison of karyotype maps within common bean 
      indicates that chromosomal distribution of the centromeric and subtelomeric 
      satellite repeats is stable, whereas the copy number of the repeats was variable, 
      indicating rapid amplification/reduction of the repeats in specific genomic 
      regions. In Phaseolus species that diverged approximately 2-4 million yr ago, 
      copy numbers of centromeric repeats were largely reduced or diverged, and 
      chromosomal distributions have changed, suggesting rapid evolution of centromeric 
      repeats. We also detected variation in the distribution pattern of subtelomeric 
      repeats in Phaseolus species. The FISH-based karyotyping system revealed that 
      satellite repeats are actively and rapidly evolving, forming genomic features 
      unique to individual common bean accessions and Phaseolus species.
CI  - Copyright (c) 2016 Iwata-Otsubo et al.
FAU - Iwata-Otsubo, Aiko
AU  - Iwata-Otsubo A
AUID- ORCID: 0000-0001-9378-3961
AD  - Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 
      30602.
FAU - Radke, Brittany
AU  - Radke B
AD  - Department of Agronomy, Purdue University, West Lafayette, Indiana 47907.
FAU - Findley, Seth
AU  - Findley S
AD  - Division of Plant Sciences, National Center for Soybean Biotechnology, University 
      of Missouri, Columbia, Missouri 65211.
FAU - Abernathy, Brian
AU  - Abernathy B
AD  - Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 
      30602.
FAU - Vallejos, C Eduardo
AU  - Vallejos CE
AD  - Department of Horticultural Sciences, University of Florida, Gainesville, Florida 
      32611.
FAU - Jackson, Scott A
AU  - Jackson SA
AD  - Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia 
      30602 sjackson@uga.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160407
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Base Sequence
MH  - Centromere/*genetics
MH  - DNA, Ribosomal/genetics
MH  - Evolution, Molecular
MH  - Genes, Plant
MH  - Genetic Variation
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - *Karyotyping
MH  - Phaseolus/classification/*genetics
MH  - Phylogeny
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Species Specificity
PMC - PMC4825637
OTO - NOTNLM
OT  - chromosome evolution
OT  - common bean
OT  - fluorescence in situ hybridization
OT  - karyotyping
OT  - satellite repeats
EDAT- 2016/02/13 06:00
MHDA- 2017/11/29 06:00
PMCR- 2016/02/09
CRDT- 2016/02/12 06:00
PHST- 2016/02/12 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/02/09 00:00 [pmc-release]
AID - g3.115.024984 [pii]
AID - GGG_024984 [pii]
AID - 10.1534/g3.115.024984 [doi]
PST - epublish
SO  - G3 (Bethesda). 2016 Apr 7;6(4):1013-22. doi: 10.1534/g3.115.024984.