PMID- 26867220
OWN - NLM
STAT- MEDLINE
DCOM- 20160725
LR  - 20201215
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - Non-Classical Monocytes and Monocyte Chemoattractant Protein-1 (MCP-1) Correlate 
      with Coronary Artery Calcium Progression in Chronically HIV-1 Infected Adults on 
      Stable Antiretroviral Therapy.
PG  - e0149143
LID - 10.1371/journal.pone.0149143 [doi]
LID - e0149143
AB  - BACKGROUND: Persistent inflammation and immune activation has been hypothesized 
      to contribute to increased prevalence of subclinical atherosclerosis and 
      cardiovascular disease (CVD) risk in patients with chronic HIV infection. In this 
      study, we examined the correlation of peripheral monocyte subsets and soluble 
      biomarkers of inflammation to coronary artery calcium (CAC) progression, as 
      measured by cardiac computed tomography scan. METHODS: We conducted a 
      longitudinal analysis utilizing baseline data of 78 participants with HIV 
      infection on stable antiretroviral therapy (ART) in the Hawaii Aging with 
      HIV-Cardiovascular study who had available baseline monocyte subset analysis as 
      well as CAC measurement at baseline and at 2-year follow up. Monocyte phenotypes 
      were assessed from cryopreserved blood by flow cytometry and plasma was assayed 
      for soluble biomarkers using antibody-coated beads in a high sensitivity 
      Milliplex Luminex platform. Change in CAC over 2 years was analyzed as the 
      primary outcome variable. RESULTS: Of all monocyte subsets and biomarkers tested, 
      higher non-classical monocyte percentage (rho = 0.259, p = 0.022), interleukin 
      (IL)-6 (rho = 0.311, p = 0.012), and monocyte chemoattractant protein (MCP)-1 (rho = 
      0.524, p = <0.001) were significantly correlated to higher 2-year CAC progression 
      in unadjusted Spearman's correlation. Non-classical monocyte percentage (rho = 
      0.247, p = 0.039), and MCP-1 (rho = 0.487, p = <0.001), remained significantly 
      correlated to 2-year CAC progression, while IL-6 was not (rho = 0.209, p = 0.120) 
      after adjustment for age, hypertension, diabetes mellitus, total/HDL cholesterol 
      ratio, smoking history, and BMI. CONCLUSION: The percentage of non-classical 
      monocytes and plasma MCP-1 levels were independently associated with CAC 
      progression and may be related to the progression of atherosclerosis and 
      increased CVD risk associated with chronic HIV infection on stable ART.
FAU - Zungsontiporn, Nath
AU  - Zungsontiporn N
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
FAU - Tello, Raquel R
AU  - Tello RR
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
FAU - Zhang, Guangxiang
AU  - Zhang G
AD  - Department of Tropical Medicine, University of Hawaii John A. Burns School of 
      Medicine, Honolulu, Hawaii, United States of America.
FAU - Mitchell, Brooks I
AU  - Mitchell BI
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
AD  - Department of Tropical Medicine, University of Hawaii John A. Burns School of 
      Medicine, Honolulu, Hawaii, United States of America.
FAU - Budoff, Matthew
AU  - Budoff M
AD  - Los Angeles Biomedical Research Institute at Harbor-UCLA, Los Angeles, 
      California, United States of America.
FAU - Kallianpur, Kalpana J
AU  - Kallianpur KJ
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
FAU - Nakamoto, Beau K
AU  - Nakamoto BK
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
AD  - Straub Hospital, Honolulu, Hawaii, United States of America.
FAU - Keating, Sheila M
AU  - Keating SM
AD  - Blood Systems Research Institute, San Francisco, California, United States of 
      America.
FAU - Norris, Philip J
AU  - Norris PJ
AD  - Blood Systems Research Institute, San Francisco, California, United States of 
      America.
AD  - Department of Laboratory Medicine, University of California, San Francisco, 
      California, United States of America.
AD  - Department of Medicine, University of California, San Francisco, California, 
      United States of America.
FAU - Ndhlovu, Lishomwa C
AU  - Ndhlovu LC
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
AD  - Department of Tropical Medicine, University of Hawaii John A. Burns School of 
      Medicine, Honolulu, Hawaii, United States of America.
FAU - Souza, Scott A
AU  - Souza SA
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
FAU - Shikuma, Cecilia M
AU  - Shikuma CM
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
FAU - Chow, Dominic C
AU  - Chow DC
AD  - Hawaii Center for AIDS, Department of Medicine, University of Hawaii John A. 
      Burns School of Medicine, Honolulu, Hawaii, United States of America.
LA  - eng
GR  - R01HL095135/HL/NHLBI NIH HHS/United States
GR  - U54 MD007584/MD/NIMHD NIH HHS/United States
GR  - R01 HL095135/HL/NHLBI NIH HHS/United States
GR  - K23 HL088981/HL/NHLBI NIH HHS/United States
GR  - U54MD007584/MD/NIMHD NIH HHS/United States
GR  - K23HL088981/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160211
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Anti-Retroviral Agents)
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Aging
MH  - Anti-Retroviral Agents/therapeutic use
MH  - Biomarkers/blood
MH  - Calcinosis/physiopathology
MH  - Calcium/*metabolism
MH  - Cardiovascular Diseases/*complications/drug therapy
MH  - Chemokine CCL2/*blood
MH  - Comorbidity
MH  - Coronary Vessels/pathology/*physiopathology
MH  - Disease Progression
MH  - Female
MH  - HIV Infections/*blood/complications/drug therapy
MH  - HIV-1
MH  - Hawaii
MH  - Humans
MH  - Inflammation
MH  - Longitudinal Studies
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*cytology
MH  - Phenotype
MH  - Tomography, X-Ray Computed
PMC - PMC4750941
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/13 06:00
MHDA- 2016/07/28 06:00
PMCR- 2016/02/11
CRDT- 2016/02/12 06:00
PHST- 2015/11/18 00:00 [received]
PHST- 2016/01/27 00:00 [accepted]
PHST- 2016/02/12 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
PHST- 2016/02/11 00:00 [pmc-release]
AID - PONE-D-15-48598 [pii]
AID - 10.1371/journal.pone.0149143 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 11;11(2):e0149143. doi: 10.1371/journal.pone.0149143. 
      eCollection 2016.