PMID- 26868150 OWN - NLM STAT- MEDLINE DCOM- 20161031 LR - 20181202 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 207 DP - 2016 May TI - The shift of the microbial community in activated sludge with calcium treatment and its implication to sludge settleability. PG - 11-8 LID - S0960-8524(16)30111-0 [pii] LID - 10.1016/j.biortech.2016.01.135 [doi] AB - The sludge settleability is of prime importance for the activated sludge process. The effect of calcium ion on the biological performance of sludge was investigated in a lab-scale activated sludge system with varying Ca(2+) concentration. Results indicated that addition of 150mg/L Ca(2+) to the influent significantly improved the settling characteristics and metabolic reactivity of activated sludge in the bioreactors. Analyses using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA sequencing showed that a significant difference in the presence of certain bacterial groups between the sludge systems with 150mg/L Ca(2+) and those with 0-100mg/L Ca(2+) addition. Ca(2+) also increased the production of the extracellular polymeric substance (EPS) and facilitated the development of microbial cluster in the bioreactor. Study showed that an addition of 150mg/L Ca(2+) to the influent provides a simple approach to improve the settling properties of activated sludge and maintain high pollutant removal efficiency. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Ye, Chengchen AU - Ye C AD - Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. FAU - Yang, Xinping AU - Yang X AD - Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xpyang@njau.edu.cn. FAU - Zhao, Fang-Jie AU - Zhao FJ AD - Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK. FAU - Ren, Lifei AU - Ren L AD - Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160208 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Polysaccharides, Bacterial) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Sewage) RN - S88TT14065 (Oxygen) RN - SY7Q814VUP (Calcium) SB - IM MH - Bacteria/*drug effects/genetics MH - Bioreactors/microbiology MH - Calcium/*pharmacology MH - Cluster Analysis MH - Denaturing Gradient Gel Electrophoresis MH - Hydrogen-Ion Concentration MH - Oxygen/analysis MH - Phylogeny MH - Polysaccharides, Bacterial/metabolism MH - RNA, Ribosomal, 16S/genetics MH - Sewage/*microbiology OTO - NOTNLM OT - Activated sludge OT - Ca(2+) OT - EPS OT - Microbial community OT - Settleability EDAT- 2016/02/13 06:00 MHDA- 2016/11/01 06:00 CRDT- 2016/02/13 06:00 PHST- 2015/12/11 00:00 [received] PHST- 2016/01/27 00:00 [revised] PHST- 2016/01/31 00:00 [accepted] PHST- 2016/02/13 06:00 [entrez] PHST- 2016/02/13 06:00 [pubmed] PHST- 2016/11/01 06:00 [medline] AID - S0960-8524(16)30111-0 [pii] AID - 10.1016/j.biortech.2016.01.135 [doi] PST - ppublish SO - Bioresour Technol. 2016 May;207:11-8. doi: 10.1016/j.biortech.2016.01.135. Epub 2016 Feb 8.