PMID- 26868822
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 414
IP  - 1-2
DP  - 2016 Mar
TI  - Induction of hypoxia-inducible factor-1alpha by BDNF protects retinoblastoma cells 
      against chemotherapy-induced apoptosis.
PG  - 77-84
LID - 10.1007/s11010-016-2660-y [doi]
AB  - Alternations of environment signals such as neurotrophins may be the basis for 
      malignant transformation of retinoblastoma (Rb), the most common primary 
      intraocular malignancy in children. The aim of this study is to investigate the 
      ability of brain-derived neurotrophic factor (BDNF) to decrease the 
      chemosensitivity of Rb cells to the common chemotherapeutic agents and to explore 
      the role of hypoxia-inducible factor-1alpha (HIF-1alpha) in such cellular process. The 
      results showed that BDNF could induce higher expression of HIF-1alpha via activation 
      of TrkB in human Y-79 retinoblastoma cells, which consequently contributed to its 
      effect against chemotherapeutic agent-induced cytotoxicity and cell apoptosis. 
      However, this protective effect could be potently reversed by knockdown of 
      HIF-1alpha. Furthermore, BDNF strikingly prevented chemotherapeutic agent-induced 
      alternations of apoptosis-related molecules, which could also be attenuated by 
      silencing HIF-1alpha. Therefore, our findings demonstrated that BDNF could contribute 
      to chemoresistance of Rb via modulation of HIF-1alpha expression, indicating that 
      targeting at the BDNF-TrkB/HIF-1alpha signaling pathway might be a promising strategy 
      for the treatment of retinoblastoma in the future.
FAU - Gao, Yu
AU  - Gao Y
AD  - Department of Ophthalmology, No. 411 Hospital of CPLA, Shanghai, 200081, China.
FAU - Jing, Ming
AU  - Jing M
AD  - Department of Ophthalmology, No. 411 Hospital of CPLA, Shanghai, 200081, China.
FAU - Ge, Rongrong
AU  - Ge R
AD  - Department of Ophthalmology, No. 411 Hospital of CPLA, Shanghai, 200081, China.
FAU - Lang, Lili
AU  - Lang L
AD  - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, 200081, China. lililang001@outlook.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160211
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Membrane Glycoproteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (tropomyosin-related kinase-B, human)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*physiology
MH  - Cell Line, Tumor
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*biosynthesis
MH  - Membrane Glycoproteins/metabolism
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Receptor, trkB
MH  - Retinoblastoma/*pathology
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Chemotherapy resistance
OT  - Hypoxia-inducible factor-1alpha
OT  - Retinoblastoma
EDAT- 2016/02/13 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/13 06:00
PHST- 2015/11/09 00:00 [received]
PHST- 2016/02/03 00:00 [accepted]
PHST- 2016/02/13 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s11010-016-2660-y [pii]
AID - 10.1007/s11010-016-2660-y [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2016 Mar;414(1-2):77-84. doi: 10.1007/s11010-016-2660-y. Epub 
      2016 Feb 11.