PMID- 26869715
OWN - NLM
STAT- MEDLINE
DCOM- 20170119
LR  - 20190104
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 40
IP  - 4
DP  - 2016 May
TI  - Improved Chiral Separation of Methamphetamine Enantiomers Using CSP-LC-MS-MS.
PG  - 255-63
LID - 10.1093/jat/bkw005 [doi]
AB  - To determine the true enantiomeric composition of methamphetamine urine drug 
      testing results, chiral separation of dextro (D) and levo (L) enantiomers is 
      necessary. While enantiomeric separation of methamphetamine has traditionally 
      been accomplished using gas chromatography-mass spectrometry (GC-MS), chiral 
      separation of D- and L-methamphetamine by chiral stationary phase (CSP) liquid 
      chromatography-mass spectrometry/mass spectrometry (LC-MS-MS) has proved more 
      reliable. Chirally selective detection of methamphetamine by GC-MS is often 
      performed using L-N-trifluoroacetyl-prolyl chloride (TPC). L-TPC, a chiral 
      compound, is known to have impurities that can affect the chiral composition 
      percentages of the methamphetamine sample, potentially leading to inaccurate 
      patient results. The comparative analysis of the samples run by GC and LC methods 
      showed preferential bias of the GC method for producing error rates, consistent 
      with previous research, of 8-19%. The CSP-LC-MS-MS method produces percent 
      deviation errors of <2%. Additionally, the GC method failed to produce results 
      that were 100% D- or L-isomer even for enantiomerically pure standards. A higher 
      rate of D- and L-methamphetamine isomer racemization is seen in samples when 
      analyzed by GC-MS using L-TPC-derivatizing agent. This racemization is not seen 
      when these samples are tested with CSP-LC-MS-MS. Thus, a more accurate method of 
      enantiomeric analysis is provided by CSP-LC-MS-MS.
CI  - (c) The Author 2016. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Ward, Lauren F
AU  - Ward LF
AD  - Ameritox Ltd, 486 Gallimore Dairy Road, Greensboro, NC 27409, USA 
      lauren.ward@ameritox.com.
FAU - Enders, Jeffrey R
AU  - Enders JR
AD  - Ameritox Ltd, 486 Gallimore Dairy Road, Greensboro, NC 27409, USA.
FAU - Bell, David S
AU  - Bell DS
AD  - Sigma-Aldrich/Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA.
FAU - Cramer, Hugh M
AU  - Cramer HM
AD  - Sigma-Aldrich/Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA.
FAU - Wallace, Frank N
AU  - Wallace FN
AD  - Ameritox Ltd, 486 Gallimore Dairy Road, Greensboro, NC 27409, USA.
FAU - McIntire, Gregory L
AU  - McIntire GL
AD  - Ameritox Ltd, 486 Gallimore Dairy Road, Greensboro, NC 27409, USA.
LA  - eng
PT  - Journal Article
DEP - 20160210
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Fluoroacetates)
RN  - 0 (Indicators and Reagents)
RN  - 44RAL3456C (Methamphetamine)
RN  - A23U71SY9G (trifluoroacetyl chloride)
SB  - IM
MH  - Calibration
MH  - Chromatography, Gas
MH  - Chromatography, High Pressure Liquid
MH  - Fluoroacetates/chemistry
MH  - Humans
MH  - Indicators and Reagents
MH  - Limit of Detection
MH  - Methamphetamine/chemistry/*isolation & purification/urine
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
MH  - Stereoisomerism
MH  - Tandem Mass Spectrometry
EDAT- 2016/02/13 06:00
MHDA- 2017/01/20 06:00
CRDT- 2016/02/13 06:00
PHST- 2016/02/13 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2017/01/20 06:00 [medline]
AID - bkw005 [pii]
AID - 10.1093/jat/bkw005 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2016 May;40(4):255-63. doi: 10.1093/jat/bkw005. Epub 2016 Feb 10.