PMID- 26869823
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160212
LR  - 20200930
IS  - 1226-8453 (Print)
IS  - 2093-4947 (Electronic)
IS  - 1226-8453 (Linking)
VI  - 39
IP  - 4
DP  - 2015 Oct
TI  - Ginsenoside Re inhibits pacemaker potentials via adenosine triphosphate-sensitive 
      potassium channels and the cyclic guanosine monophosphate/nitric oxide-dependent 
      pathway in cultured interstitial cells of Cajal from mouse small intestine.
PG  - 314-21
LID - 10.1016/j.jgr.2015.02.004 [doi]
AB  - BACKGROUND: Ginseng belongs to the genus Panax. Its main active ingredients are 
      the ginsenosides. Interstitial cells of Cajal (ICCs) are the pacemaker cells of 
      the gastrointestinal (GI) tract. To understand the effects of ginsenoside Re 
      (GRe) on GI motility, the authors investigated its effects on the pacemaker 
      activity of ICCs of the murine small intestine. METHODS: Interstitial cells of 
      Cajal were dissociated from mouse small intestines by enzymatic digestion. The 
      whole-cell patch clamp configuration was used to record pacemaker potentials in 
      cultured ICCs. Changes in cyclic guanosine monophosphate (cGMP) content induced 
      by GRe were investigated. RESULTS: Ginsenoside Re (20-40muM) decreased the 
      amplitude and frequency of ICC pacemaker activity in a concentration-dependent 
      manner. This action was blocked by guanosine 5'-[beta-thio]diphosphate [a 
      guanosine-5'-triphosphate (GTP)-binding protein inhibitor] and by glibenclamide 
      [an adenosine triphosphate (ATP)-sensitive K(+) channel blocker]. To study the 
      GRe-induced signaling pathway in ICCs, the effects of 
      1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (a guanylate cyclase inhibitor) and 
      RP-8-CPT-cGMPS (a protein kinase G inhibitor) were examined. Both inhibitors 
      blocked the inhibitory effect of GRe on ICC pacemaker activity. 
      L-NG-nitroarginine methyl ester (100muM), which is a nonselective nitric oxide 
      synthase (NOS) inhibitor, blocked the effects of GRe on ICC pacemaker activity 
      and GRe-stimulated cGMP production in ICCs. CONCLUSION: In cultured murine ICCs, 
      GRe inhibits the pacemaker activity of ICCs via the ATP-sensitive potassium 
      (K(+)) channel and the cGMP/NO-dependent pathway. Ginsenoside Re may be a basis 
      for developing novel spasmolytic agents to prevent or alleviate GI motility 
      dysfunction.
FAU - Hong, Noo Ri
AU  - Hong NR
AD  - Division of Longevity and Biofunctional Medicine, Pusan National University 
      School of Korean Medicine, Yangsan, Korea; Healthy Aging Korean Medical Research 
      Center, Pusan National University School of Korean Medicine, Yangsan, Korea.
FAU - Park, Hyun Soo
AU  - Park HS
AD  - Division of Longevity and Biofunctional Medicine, Pusan National University 
      School of Korean Medicine, Yangsan, Korea; Healthy Aging Korean Medical Research 
      Center, Pusan National University School of Korean Medicine, Yangsan, Korea.
FAU - Ahn, Tae Seok
AU  - Ahn TS
AD  - Division of Longevity and Biofunctional Medicine, Pusan National University 
      School of Korean Medicine, Yangsan, Korea; Healthy Aging Korean Medical Research 
      Center, Pusan National University School of Korean Medicine, Yangsan, Korea.
FAU - Kim, Hyun Jung
AU  - Kim HJ
AD  - Division of Longevity and Biofunctional Medicine, Pusan National University 
      School of Korean Medicine, Yangsan, Korea; Healthy Aging Korean Medical Research 
      Center, Pusan National University School of Korean Medicine, Yangsan, Korea.
FAU - Ha, Ki-Tae
AU  - Ha KT
AD  - Healthy Aging Korean Medical Research Center, Pusan National University School of 
      Korean Medicine, Yangsan, Korea; Division of Applied Medicine, School of Korean 
      Medicine, Pusan National University, Yangsan, Korea.
FAU - Kim, Byung Joo
AU  - Kim BJ
AD  - Division of Longevity and Biofunctional Medicine, Pusan National University 
      School of Korean Medicine, Yangsan, Korea; Healthy Aging Korean Medical Research 
      Center, Pusan National University School of Korean Medicine, Yangsan, Korea.
LA  - eng
PT  - Journal Article
DEP - 20150306
PL  - Korea (South)
TA  - J Ginseng Res
JT  - Journal of ginseng research
JID - 100890690
PMC - PMC4593795
OTO - NOTNLM
OT  - gastrointestinal tract
OT  - ginsenoside Re
OT  - interstitial cells of Cajal
OT  - patch clamp configuration
EDAT- 2016/02/13 06:00
MHDA- 2016/02/13 06:01
PMCR- 2015/03/06
CRDT- 2016/02/13 06:00
PHST- 2014/12/08 00:00 [received]
PHST- 2015/01/19 00:00 [revised]
PHST- 2015/02/25 00:00 [accepted]
PHST- 2016/02/13 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2016/02/13 06:01 [medline]
PHST- 2015/03/06 00:00 [pmc-release]
AID - S1226-8453(15)00014-7 [pii]
AID - 10.1016/j.jgr.2015.02.004 [doi]
PST - ppublish
SO  - J Ginseng Res. 2015 Oct;39(4):314-21. doi: 10.1016/j.jgr.2015.02.004. Epub 2015 
      Mar 6.