PMID- 26872375
OWN - NLM
STAT- MEDLINE
DCOM- 20171201
LR  - 20220408
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 7
IP  - 10
DP  - 2016 Mar 8
TI  - Regulation of laryngeal squamous cell cancer progression by the lncRNA 
      H19/miR-148a-3p/DNMT1 axis.
PG  - 11553-66
LID - 10.18632/oncotarget.7270 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a highly aggressive malignant cancer. 
      The regulation of LSCC progression by long non-coding RNA (lncRNA) was not well 
      understood. In this study, we reported that the lncRNA H19 was upregulated in 
      LSCC. The expression levels of H19 were inversely correlated with the survival 
      rate of LSCC patients. Knockdown of H19 expression inhibited LSCC cell migration, 
      invasion and proliferation. We identified microRNA miR-148a-3p as an inhibitory 
      target for H19. Overexpression of miR-148a-3p reduced LSCC migration, invasion 
      and proliferation cell, while inhibition of miR-148a-3p did the opposite. The 
      inhibition of LSCC progression induced by H19 knockdown required the activity of 
      miR-148a-3p. We also identified DNA methyltransferase enzyme DNMT1 as a target of 
      miR-148a-3p. Cellular DNA methylation levels were inhibited by both miR-148a-3p 
      overexpression and H19 knockdown. In summary, our study demonstrated that the 
      lncRNA H19 promoted LSCC progression via miR-148a-3p and DNMT1.
FAU - Wu, Tianyi
AU  - Wu T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Qu, Lingmei
AU  - Qu L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Fifth Affiliated 
      Hospital of Harbin Medical University, Daqing, China.
FAU - He, Guoqing
AU  - He G
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Li, Liang
AU  - Li L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Zhou, Han
AU  - Zhou H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Jin, Qian
AU  - Jin Q
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Ren, Jingyuan
AU  - Ren J
AD  - Department of Head and Neck Surgery, The Oncology Hospital of Jilin province, 
      Changchun, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Wang, Jingting
AU  - Wang J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Kan, Xuan
AU  - Kan X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Shen, Jia
AU  - Shen J
AD  - Department of Orthopaedic Surgery and The Orthopaedic Hospital Research Center, 
      University of California, Los Angeles, California, USA.
FAU - Guo, Mian
AU  - Guo M
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (H19 long non-coding RNA)
RN  - 0 (MIRN148 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - DNA (Cytosine-5-)-Methyltransferase 1/genetics/*metabolism
MH  - DNA Methylation
MH  - Disease Progression
MH  - Female
MH  - HEK293 Cells
MH  - Head and Neck Neoplasms/genetics/*metabolism/pathology
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Survival Analysis
MH  - Transfection
MH  - Up-Regulation
PMC - PMC4905493
OTO - NOTNLM
OT  - DNMT1
OT  - laryngeal squamous cell cancer
OT  - lncRNA H19
OT  - miR-148a-3p
COIS- CONFLICTS OF INTEREST The authors declare no potential conflicts of interest.
EDAT- 2016/02/13 06:00
MHDA- 2017/12/02 06:00
PMCR- 2016/03/08
CRDT- 2016/02/13 06:00
PHST- 2015/07/07 00:00 [received]
PHST- 2015/12/05 00:00 [accepted]
PHST- 2016/02/13 06:00 [entrez]
PHST- 2016/02/13 06:00 [pubmed]
PHST- 2017/12/02 06:00 [medline]
PHST- 2016/03/08 00:00 [pmc-release]
AID - 7270 [pii]
AID - 10.18632/oncotarget.7270 [doi]
PST - ppublish
SO  - Oncotarget. 2016 Mar 8;7(10):11553-66. doi: 10.18632/oncotarget.7270.