PMID- 26872561
OWN - NLM
STAT- MEDLINE
DCOM- 20171205
LR  - 20180117
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 60
IP  - 5
DP  - 2016 May
TI  - Urolithin A, C, and D, but not iso-urolithin A and urolithin B, attenuate 
      triglyceride accumulation in human cultures of adipocytes and hepatocytes.
PG  - 1129-38
LID - 10.1002/mnfr.201500796 [doi]
AB  - SCOPE: Urolithins (Uro) are ellagic acid (EA)-derived metabolites produced by gut 
      microbes. There is a growing interest in the biological activities of Uro. Our 
      aim was to evaluate the impacts of Uro on regulating triglyceride (TG) 
      accumulation using cultures of primary human adipocytes and hepatoma Huh7 cells. 
      METHODS AND RESULTS: UroA, UroB, UroC, UroD, and iso-UroA were used to determine 
      the effect of Uro on adipogenesis and lipogenesis. Individual Uro (30 muM) were 
      added to human adipogenic stem cells during differentiation. UroA, UroC, and 
      UroD, but not iso-UroA and UroB, significantly inhibited new fat cell formation 
      by decreasing TG accumulation and adipogenic protein and gene expressions. The 
      regulation of TG synthesis by Uro was investigated via metabolic chasing with 
      radiolabeled precursors. UroA, UroC, and UroD attenuated TG accumulation, while 
      increasing the fatty acid (FA) oxidation in adipocytes and h/epatoma Huh7 cells. 
      Furthermore, UroC, UroD, and UroA promoted the phosphorylation of AMP-activated 
      protein kinase, suggesting that Uro may alter energy-sensing metabolic pathways 
      in primary human adipocytes. CONCLUSIONS: Taken together, our results 
      demonstrated that UroA, UroC, and UroD, but not isoUroA and UroB, reduce TG 
      accumulation and increase FA oxidation in adipocytes as well as hepatocytes.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Kang, Inhae
AU  - Kang I
AD  - Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 
      Lincoln, NE, USA.
FAU - Kim, YongEun
AU  - Kim Y
AD  - Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 
      Lincoln, NE, USA.
FAU - Tomas-Barberan, Francisco A
AU  - Tomas-Barberan FA
AD  - Department of Food Science and Technology, CEBAS-CSIC, Murcia, Spain.
FAU - Espin, Juan Carlos
AU  - Espin JC
AD  - Department of Food Science and Technology, CEBAS-CSIC, Murcia, Spain.
FAU - Chung, Soonkyu
AU  - Chung S
AD  - Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, 
      Lincoln, NE, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160413
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Coumarins)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (Triglycerides)
RN  - 0 (urolithin B)
RN  - 0 (urolithin C)
RN  - 0 (urolithin D)
RN  - 1143-70-0 (3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one)
SB  - IM
MH  - Adipocytes/*drug effects/metabolism
MH  - Adipogenesis/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Coumarins/*pharmacology
MH  - Hepatocytes/*drug effects/metabolism
MH  - Humans
MH  - Hydrolyzable Tannins/*pharmacology
MH  - Lipid Metabolism/drug effects
MH  - Triglycerides/metabolism
OTO - NOTNLM
OT  - AMP-activated protein kinase
OT  - Adipogenesis
OT  - Ellagic acid
OT  - Lipogenesis
OT  - Urolithins
EDAT- 2016/02/14 06:00
MHDA- 2017/12/06 06:00
CRDT- 2016/02/14 06:00
PHST- 2015/10/09 00:00 [received]
PHST- 2016/01/30 00:00 [revised]
PHST- 2016/02/03 00:00 [accepted]
PHST- 2016/02/14 06:00 [entrez]
PHST- 2016/02/14 06:00 [pubmed]
PHST- 2017/12/06 06:00 [medline]
AID - 10.1002/mnfr.201500796 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2016 May;60(5):1129-38. doi: 10.1002/mnfr.201500796. Epub 2016 
      Apr 13.