PMID- 26873249
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Linking)
VI  - 68
IP  - 4
DP  - 2016 Apr
TI  - Explore the variation of MMP3, JNK, p38 MAPKs, and autophagy at the early stage 
      of osteoarthritis.
PG  - 293-302
LID - 10.1002/iub.1482 [doi]
AB  - Osteoarthritis is a chronic disease characterized by cartilage degeneration and 
      chondrocyte apoptosis. Mitogen-activated protein kinase (MAPK) signaling pathway 
      plays a key role in regulating OA process. Autophagy has an important effect on 
      the OA process, and it is believed to be regulated by MAPKs. To reveal the 
      mechanism and the effect of JNK and p38 MAPKs on matrix metalloproteinase 3 
      (MMP3) and autophagy in OA, the study established OA model in rabbits, used the 
      measurement of the Osteoarthritis Research Society International scoring system 
      to evaluate OA model, and conducted general observation, histological 
      observation, and Western blotting of JNK, phosphorylate-JNK (P-JNK), p38, 
      phosphorylate-p38 (P-p38), MMP3, and light-chain 3 (LC3)-II/LC3-I to explore the 
      variation of JNK, p38 MAPKs, and autophagy at the early stage of OA. With OA 
      progressing at the early stage, MMP3, P-p38, and P-JNK were gradually upregulated 
      from the baseline to the peak in study groups when compared with the control 
      group; JNK and p38 variated of turbulence without statistical difference; and 
      LC3-II/LC3-I had a decreasing tendency from the 0- to 15-day group. This study 
      identifies that compromised autophagy may be related to the OA progress and that 
      JNK and p38 MAPKs have positive regulation on MMP3 and negative regulation on 
      autophagy. It also implicates a new therapeutic strategy for OA and other 
      degenerate diseases based on selective MAPK inhibitors, reduction of MMP3, and 
      autophagy.
CI  - (c) 2016 International Union of Biochemistry and Molecular Biology.
FAU - Shi, Jie
AU  - Shi J
AD  - Department of Rehabilitation Medicine, The Second Xiangya Hospital of Central 
      South University, Changsha, Hunan, People's Republic of China.
FAU - Zhang, Changjie
AU  - Zhang C
AD  - Department of Rehabilitation Medicine, The Second Xiangya Hospital of Central 
      South University, Changsha, Hunan, People's Republic of China.
FAU - Yi, Zhongjie
AU  - Yi Z
AD  - Department of General Surgery, The Third Xiangya Hospital of Central South 
      University, Changsha, Hunan, People's Republic of China.
FAU - Lan, Chunna
AU  - Lan C
AD  - Department of Rehabilitation Medicine, The Second Xiangya Hospital of Central 
      South University, Changsha, Hunan, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20160213
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Microtubule-Associated Proteins)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Autophagy/*genetics
MH  - Cartilage, Articular/metabolism/pathology
MH  - Chondrocytes/*metabolism/pathology
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Gene Expression Regulation
MH  - Histocytochemistry
MH  - Humans
MH  - MAP Kinase Kinase 4/*genetics/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Microtubule-Associated Proteins/genetics/metabolism
MH  - Osteoarthritis/*genetics/metabolism/pathology
MH  - Phosphorylation
MH  - Rabbits
MH  - Signal Transduction
MH  - p38 Mitogen-Activated Protein Kinases/*genetics/metabolism
OTO - NOTNLM
OT  - JNK
OT  - LC3
OT  - MAPK
OT  - autophagy
OT  - osteoarthritis
OT  - p38
OT  - signaling pathway
EDAT- 2016/02/14 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/14 06:00
PHST- 2015/12/12 00:00 [received]
PHST- 2016/01/17 00:00 [accepted]
PHST- 2016/02/14 06:00 [entrez]
PHST- 2016/02/14 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1002/iub.1482 [doi]
PST - ppublish
SO  - IUBMB Life. 2016 Apr;68(4):293-302. doi: 10.1002/iub.1482. Epub 2016 Feb 13.