PMID- 26874236
OWN - NLM
STAT- MEDLINE
DCOM- 20170315
LR  - 20171116
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 77
IP  - 9
DP  - 2016 Sep
TI  - Multiplex bead-based immunoassay for the free soluble forms of the HLA-G 
      receptors, ILT2 and ILT4.
PG  - 720-6
LID - S0198-8859(16)00026-4 [pii]
LID - 10.1016/j.humimm.2016.01.017 [doi]
AB  - Human leukocyte antigen (HLA)-G is an immune-inhibitory molecule that exerts its 
      function via interaction with two main inhibitory receptors: ILT2 and ILT4. This 
      interaction is considered to be an immune checkpoint. HLA-G can be found as a 
      soluble molecule, but it is not known if its receptors can also be found as 
      soluble molecules. In this work, we present a multiplex luminex-based assay to 
      measure soluble ILT2 (sILT2) and soluble ILT4 (sILT4) molecules together. It is 
      based on two antibody pairs, GHI/75 and HP-F1-PE for ILT2 and 27D6 and 42D1-PE 
      for ILT4. The characterization of our method reveals that it specifically detects 
      the free soluble forms of sILT2 and sILT4, and not those complexed to HLA Class I 
      molecules such as their ligand of highest affinity HLA-G. A study on two small 
      cohorts of cancer patients demonstrated that soluble ILT2 and ILT4 molecules were 
      of low abundance in the plasma of healthy controls, but that elevated levels of 
      plasmatic sILT2 were present in non-muscle-infiltrating bladder cancer patients. 
      This demonstrated that the titration test is indeed working, and that soluble 
      ILT2 molecules do exist in pathological contexts, which relevance may now be 
      sought on larger cohorts and other pathologies.
CI  - Copyright (c) 2016. Published by Elsevier Inc.
FAU - Wu, Ching-Lien
AU  - Wu CL
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, 1 Avenue Claude 
      Vellefaux, Bat Lailler, 75475 Paris Cedex 10, France; University Paris Diderot, 
      Sorbonne Paris Cite, UMR E_5 Institut Universitaire d'Hematologie, Saint-Louis 
      Hospital, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France.
FAU - Svendsen, Signe Goul
AU  - Svendsen SG
AD  - Department of Immunology and Microbiology, University of Copenhagen, Blegdamsvej 
      3, 2200 Copenhagen N, Denmark.
FAU - Riviere, Adrien
AU  - Riviere A
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, 1 Avenue Claude 
      Vellefaux, Bat Lailler, 75475 Paris Cedex 10, France; University Paris Diderot, 
      Sorbonne Paris Cite, UMR E_5 Institut Universitaire d'Hematologie, Saint-Louis 
      Hospital, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France; AP-HP, 
      Saint-Louis Hospital, Department of Urology, 1 Avenue Claude Vellefaux, 75475 
      Paris Cedex 10, France.
FAU - Desgrandchamps, Francois
AU  - Desgrandchamps F
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, 1 Avenue Claude 
      Vellefaux, Bat Lailler, 75475 Paris Cedex 10, France; AP-HP, Saint-Louis 
      Hospital, Department of Urology, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, 
      France.
FAU - Carosella, Edgardo D
AU  - Carosella ED
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, 1 Avenue Claude 
      Vellefaux, Bat Lailler, 75475 Paris Cedex 10, France; University Paris Diderot, 
      Sorbonne Paris Cite, UMR E_5 Institut Universitaire d'Hematologie, Saint-Louis 
      Hospital, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France.
FAU - LeMaoult, Joel
AU  - LeMaoult J
AD  - Hemato-Immunology Research Department, CEA, Hospital Saint Louis, 1 Avenue Claude 
      Vellefaux, Bat Lailler, 75475 Paris Cedex 10, France; University Paris Diderot, 
      Sorbonne Paris Cite, UMR E_5 Institut Universitaire d'Hematologie, Saint-Louis 
      Hospital, 1 Avenue Claude Vellefaux, 75475 Paris Cedex 10, France. Electronic 
      address: joel.lemoult@cea.fr.
LA  - eng
PT  - Journal Article
DEP - 20160210
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, CD)
RN  - 0 (Blood Proteins)
RN  - 0 (HLA-G Antigens)
RN  - 0 (LILRB1 protein, human)
RN  - 0 (LILRB2 protein, human)
RN  - 0 (Leukocyte Immunoglobulin-like Receptor B1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Blood Proteins/*metabolism
MH  - Cohort Studies
MH  - HLA-G Antigens/metabolism
MH  - Humans
MH  - Immunomagnetic Separation/*methods
MH  - Leukocyte Immunoglobulin-like Receptor B1
MH  - Membrane Glycoproteins/*metabolism
MH  - Receptors, Immunologic/*metabolism
MH  - Urinary Bladder Neoplasms/*diagnosis
OTO - NOTNLM
OT  - Human leukocyte antigen G (HLA-G)
OT  - ILT2
OT  - ILT4
OT  - Immune check point
OT  - Immune regulation
OT  - Immunoassay
EDAT- 2016/02/14 06:00
MHDA- 2017/03/16 06:00
CRDT- 2016/02/14 06:00
PHST- 2015/10/16 00:00 [received]
PHST- 2016/01/20 00:00 [revised]
PHST- 2016/01/21 00:00 [accepted]
PHST- 2016/02/14 06:00 [entrez]
PHST- 2016/02/14 06:00 [pubmed]
PHST- 2017/03/16 06:00 [medline]
AID - S0198-8859(16)00026-4 [pii]
AID - 10.1016/j.humimm.2016.01.017 [doi]
PST - ppublish
SO  - Hum Immunol. 2016 Sep;77(9):720-6. doi: 10.1016/j.humimm.2016.01.017. Epub 2016 
      Feb 10.