PMID- 26874342
OWN - NLM
STAT- MEDLINE
DCOM- 20170208
LR  - 20220321
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 66
DP  - 2016 Jun
TI  - The expression of human beta-defensins (hBD-1, hBD-2, hBD-3, hBD-4) in gingival 
      epithelia.
PG  - 15-21
LID - S0003-9969(16)30012-7 [pii]
LID - 10.1016/j.archoralbio.2016.01.012 [doi]
AB  - OBJECTIVE: The aim of this study was to analyze and compare the expression 
      patterns of human beta-defensin-4 (hBD-4) with those of hBD-1, hBD-2, and hBD-3 in 
      healthy and chronically inflamed gingival tissues. MATERIALS AND METHODS: A total 
      of 96 gingival samples were collected, comprising 46 biopsies from chronic 
      periodontitis (CP) patients and 50 from individuals with healthy tissue. Of 
      these, 21CP and 21 healthy samples were used to examine the protein expression of 
      the hBDs by immunohistochemistry. The remaining 25CP and 29 healthy tissue 
      samples were subjected to real-time reverse transcription polymerase chain 
      reaction to quantify the mRNA expression of the hBDs. RESULTS: All four types of 
      beta-defensin peptides were confined to the gingival epithelia. The percentage of 
      samples positive for hBD-4 mRNA was significantly lower than the percentages for 
      the other three beta-defensins, both in healthy (p=0.003) and in inflamed (p=0.030) 
      gingiva. However, in the case of the relative mRNA expression levels, that of 
      hBD-4 was significantly higher than the levels for hBD-2 (healthy group: p<0.01; 
      CP group: p<0.01) and hBD-3 (healthy group: p=0.004; CP group: p<0.01). 
      CONCLUSIONS: Our study demonstrates differential expression of hBD-1, hBD-2, 
      hBD-3, and hBD-4 in healthy and CP gingiva.
CI  - Copyright (c) 2016. Published by Elsevier Ltd.
FAU - Li, Xinyi
AU  - Li X
AD  - State Key Laboratory of Oral diseases, Chengdu, PR China; Department of 
      Periodontology, College of Stomatology, Chongqing Medical University, Chongqing, 
      PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, 
      Chongqing, PR China.
FAU - Duan, Dingyu
AU  - Duan D
AD  - State Key Laboratory of Oral diseases, Chengdu, PR China.
FAU - Yang, Jingmei
AU  - Yang J
AD  - State Key Laboratory of Oral diseases, Chengdu, PR China.
FAU - Wang, Panpan
AU  - Wang P
AD  - State Key Laboratory of Oral diseases, Chengdu, PR China.
FAU - Han, Bo
AU  - Han B
AD  - Department of Oral Surgery, West China College of Stomatology, Sichuan 
      University, Chengdu, PR China.
FAU - Zhao, Lei
AU  - Zhao L
AD  - State Key Laboratory of Oral diseases, Chengdu, PR China; Department of 
      Periodontology, West China College of Stomatology, Sichuan University, Chengdu, 
      PR China.
FAU - Jepsen, Soren
AU  - Jepsen S
AD  - Department of Periodontology, Operative and Preventive Dentistry, University of 
      Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany. Electronic address: 
      sjepsen@uni-bonn.de.
FAU - Dommisch, Henrik
AU  - Dommisch H
AD  - Department of Periodontology, Operative and Preventive Dentistry, University of 
      Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany.
FAU - Winter, Jochen
AU  - Winter J
AD  - Department of Periodontology, Operative and Preventive Dentistry, University of 
      Bonn, Welschnonnenstrasse 17, 53111 Bonn, Germany.
FAU - Xu, Yi
AU  - Xu Y
AD  - State Key Laboratory of Oral diseases, Chengdu, PR China; Department of 
      Periodontology, West China College of Stomatology, Sichuan University, Chengdu, 
      PR China. Electronic address: schuehih@sohu.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160127
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (DEFB1 protein, human)
RN  - 0 (DEFB103A protein, human)
RN  - 0 (DEFB4A protein, human)
RN  - 0 (beta-Defensins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - Chronic Disease
MH  - Chronic Periodontitis/metabolism
MH  - Cytoplasm/metabolism
MH  - Epithelium/*metabolism/pathology
MH  - Female
MH  - Gingiva/*metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Periodontitis/metabolism/pathology
MH  - Young Adult
MH  - beta-Defensins/*biosynthesis
OTO - NOTNLM
OT  - Antimicrobial cationic peptide
OT  - Immunohistochemistry
OT  - Periodontitis
OT  - Real-time polymerase chain reaction
OT  - beta-Defensin
EDAT- 2016/02/14 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/02/14 06:00
PHST- 2015/04/02 00:00 [received]
PHST- 2016/01/10 00:00 [revised]
PHST- 2016/01/24 00:00 [accepted]
PHST- 2016/02/14 06:00 [entrez]
PHST- 2016/02/14 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - S0003-9969(16)30012-7 [pii]
AID - 10.1016/j.archoralbio.2016.01.012 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2016 Jun;66:15-21. doi: 10.1016/j.archoralbio.2016.01.012. Epub 
      2016 Jan 27.