PMID- 26875087
OWN - NLM
STAT- MEDLINE
DCOM- 20170116
LR  - 20220410
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 179
IP  - 3
DP  - 2016 Jun
TI  - Inhibition of Tumor Necrosis Factor-alpha and Interleukin-1beta Production in 
      Lipopolysaccharide-Stimulated Monocytes by Methanolic Extract of Elephantopus 
      scaber Linn and Identification of Bioactive Components.
PG  - 427-43
LID - 10.1007/s12010-016-2004-0 [doi]
AB  - The methanolic extract of E. scaber Linn was evaluated for anti-inflammatory 
      activity by determining its effects on production of pro-inflammatory cytokines 
      like Tumor necrosis factor-alpha (TNF-alpha) and Interleukin-1beta (IL-1beta) in 
      Lipopolysaccharide (LPS) stimulated monocytes. The cytotoxicity of the extract 
      was analyzed prior to the cytokine quantification assays. The extract was further 
      subjected to UPLC MS Q-TOF, for the identification of bioactive components 
      present in the crude extract. The extract was found not to be cytotoxic against 
      monocytes, and exhibited significant inhibition in the production of 
      pro-inflammatory cytokines. The presence of 34 components in the methanolic 
      extract was detected through mass spectrum analysis.
FAU - Abhimannue, Anu P
AU  - Abhimannue AP
AD  - Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, 
      Priyadarshini Hills, Kottayam, Kerala, India, 686560.
FAU - Mohan, Mohind C
AU  - Mohan MC
AD  - Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, 
      Priyadarshini Hills, Kottayam, Kerala, India, 686560.
FAU - B, Prakash Kumar
AU  - B PK
AD  - Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, 
      Priyadarshini Hills, Kottayam, Kerala, India, 686560. prakashkumar@mgu.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20160213
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Asteraceae/chemistry
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation/*drug therapy
MH  - Interleukin-1beta/*antagonists & inhibitors/biosynthesis/genetics
MH  - Lipopolysaccharides/chemistry
MH  - Monocytes/drug effects
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/biosynthesis/genetics
OTO - NOTNLM
OT  - Elephantopus scaber
OT  - Inflammation
OT  - Interleukin-1beta
OT  - Mass spectrometry
OT  - Monocytes
OT  - Pro-inflammatory cytokines
OT  - Tumor necrosis factor-alpha
EDAT- 2016/02/15 06:00
MHDA- 2017/01/17 06:00
CRDT- 2016/02/15 06:00
PHST- 2015/12/12 00:00 [received]
PHST- 2016/01/27 00:00 [accepted]
PHST- 2016/02/15 06:00 [entrez]
PHST- 2016/02/15 06:00 [pubmed]
PHST- 2017/01/17 06:00 [medline]
AID - 10.1007/s12010-016-2004-0 [pii]
AID - 10.1007/s12010-016-2004-0 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2016 Jun;179(3):427-43. doi: 10.1007/s12010-016-2004-0. 
      Epub 2016 Feb 13.