PMID- 26878990
OWN - NLM
STAT- MEDLINE
DCOM- 20161230
LR  - 20161231
IS  - 2042-6984 (Electronic)
IS  - 2042-6976 (Linking)
VI  - 6
IP  - 4
DP  - 2016 Apr
TI  - Characterization of immunoglobulin E plasma cells that are elevated in the upper 
      airway mucosa of nonatopic patients with chronic rhinosinusitis without nasal 
      polyps.
PG  - 378-84
LID - 10.1002/alr.21696 [doi]
AB  - BACKGROUND: The immunologic mechanisms driving inflammation in the upper airways 
      of patients with chronic rhinosinusitis (CRS) are poorly understood. Previous 
      studies have shown that B cells and immunoglobulin E (IgE) levels are elevated in 
      the nasal tissue of patients with atopic chronic rhinosinusitis without nasal 
      polyps (CRSsNP). However, less is known regarding B cell subsets and 
      IgE-producing plasmablasts in nonatopic CRSsNP patients. METHODS: Human blood and 
      ethmoid sinus mucosa samples were analyzed from control (n = 6) and nonatopic 
      CRSsNP (n = 11) patients. Tissue samples were evaluated using high-dimensional 
      flow cytometry. RESULTS: A population of IgE antibody secreting cells is 
      significantly increased in situ within inflamed nasal tissue of nonatopic CRSsNP 
      subjects when compared to control nasal tissue and the circulating peripheral 
      blood (p < 0.05). This IgE plasma cell population displays  approximately 90% cell surface Ig 
      lambda light chain, is mitotically active (Ki-67(+)), and displays intracellular 
      IgE expression. The predominant B cell population expressing IgE are plasmablasts 
      (CD38(high), CD138(-)) not typically found in the blood or peripheral tissue of 
      these patients. CONCLUSION: The nasal mucosa from nonatopic CRSsNP patients 
      demonstrate a significant regional spike in resident in situ IgE plasmablast 
      cells not seen in control nasal tissue or peripheral blood from the same patient. 
      The restricted expression of Ig lambda light chain in this mitotically active IgE 
      plasmablast population supports the hypothesis of aberrant B cell proliferation 
      in the context of CRS. These findings suggest the presence of a unique regional 
      immune microenvironment for B cell priming and/or selection within chronically 
      inflamed airway tissues.
CI  - (c) 2016 ARS-AAOA, LLC.
FAU - Rashan, Ali R
AU  - Rashan AR
AD  - Division of Rhinology, Department of Otolaryngology-Head and Neck Surgery, 
      Stanford University School of Medicine, Stanford, CA.
FAU - Goshn, Eliver
AU  - Goshn E
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, CA.
FAU - Peterson, Asa
AU  - Peterson A
AD  - Division of Rhinology, Department of Otolaryngology-Head and Neck Surgery, 
      Stanford University School of Medicine, Stanford, CA.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, CA.
FAU - Phillips, Meghan
AU  - Phillips M
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, CA.
FAU - Sahaf, Bita
AU  - Sahaf B
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, CA.
FAU - Herzenberg, Leonore
AU  - Herzenberg L
AD  - Department of Genetics, Stanford University School of Medicine, Stanford, CA.
FAU - Nayak, Jayakar V
AU  - Nayak JV
AD  - Division of Rhinology, Department of Otolaryngology-Head and Neck Surgery, 
      Stanford University School of Medicine, Stanford, CA.
LA  - eng
PT  - Journal Article
DEP - 20160216
PL  - United States
TA  - Int Forum Allergy Rhinol
JT  - International forum of allergy & rhinology
JID - 101550261
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Chronic Disease
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Male
MH  - Middle Aged
MH  - Nasal Mucosa/*immunology
MH  - Nasal Polyps
MH  - Plasma Cells/*immunology
MH  - Rhinitis/*immunology
MH  - Sinusitis/*immunology
OTO - NOTNLM
OT  - B cell
OT  - CD138
OT  - CD38
OT  - FACS
OT  - IgE
OT  - PBMC
OT  - chronic rhinosinusitis
OT  - flow cytometry
OT  - nasal mucosa
OT  - plasmablast
OT  - upper airway
EDAT- 2016/02/18 06:00
MHDA- 2016/12/31 06:00
CRDT- 2016/02/17 06:00
PHST- 2014/09/09 00:00 [received]
PHST- 2015/09/13 00:00 [revised]
PHST- 2015/10/11 00:00 [accepted]
PHST- 2016/02/17 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/12/31 06:00 [medline]
AID - 10.1002/alr.21696 [doi]
PST - ppublish
SO  - Int Forum Allergy Rhinol. 2016 Apr;6(4):378-84. doi: 10.1002/alr.21696. Epub 2016 
      Feb 16.