PMID- 26879207
OWN - NLM
STAT- MEDLINE
DCOM- 20161216
LR  - 20181113
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 33
IP  - 4
DP  - 2016 Apr
TI  - Ovarian kisspeptin expression is related to age and to monocyte chemoattractant 
      protein-1.
PG  - 535-43
LID - 10.1007/s10815-016-0672-x [doi]
AB  - PURPOSE: The objective of this study was to test the hypothesis that ovarian 
      kisspeptin (kiss1) and its receptor (kiss1r) expression are affected by age, 
      obesity, and the age- and obesity-related chemokine monocyte chemoattractant 
      protein-1 (MCP-1). METHODS: Ovaries from reproductive-aged and older C57BL/6J 
      mice fed normal chow (NC) or high-fat (HF) diet, ovaries from age-matched young 
      MCP-1 knockout and young control mice on NC, and finally, cumulus and mural 
      granulosa cells (GCs) from women who underwent in vitro fertilization (IVF) were 
      collected. Kiss1, kiss1r, anti-Mullerian hormone (AMH), and AMH receptor 
      (AMHR-II) messenger RNA (mRNA) expression levels were quantified using real-time 
      polymerase chain reaction (RT-PCR). RESULTS: In mouse ovaries, kiss1 and kiss1r 
      mRNA levels were significantly higher in old compared to reproductive-aged mice, 
      and diet-induced obesity did not alter kiss1 or kiss1r mRNA levels. Compared to 
      young control mice, young MCP-1 knockout mice had significantly lower ovarian 
      kiss1 mRNA but significantly higher AMH and AMHR-II mRNA levels. In human cumulus 
      GCs, kiss1r mRNA levels were positively correlated with age but not with BMI. 
      There was no expression of kiss1 mRNA in either cumulus or mural GCs. CONCLUSION: 
      These data suggest a possible age-related physiologic role for the 
      kisspeptinergic system in ovarian physiology. Additionally, the inflammatory 
      MCP-1 may be associated with kiss1 and AMH genes, which are important in 
      ovulation and folliculogenesis, respectively.
FAU - Merhi, Zaher
AU  - Merhi Z
AD  - Department of Obstetrics and Gynecology, Division of Reproductive Biology, New 
      York University School of Medicine, 180 Varick Street, sixth floor, New York, NY, 
      10014, USA. zom00@hotmail.com.
FAU - Thornton, Kimberley
AU  - Thornton K
AD  - Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology 
      and Infertility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 
      Bronx, NY, USA.
FAU - Bonney, Elizabeth
AU  - Bonney E
AD  - Department of Obstetrics, Gynecology, and Reproductive Sciences, College of 
      Medicine, University of Vermont, Burlington, VT, USA.
FAU - Cipolla, Marilyn J
AU  - Cipolla MJ
AD  - Department of Obstetrics, Gynecology, and Reproductive Sciences, College of 
      Medicine, University of Vermont, Burlington, VT, USA.
AD  - Department of Neurological Sciences, College of Medicine, University of Vermont, 
      Burlington, VT, USA.
FAU - Charron, Maureen J
AU  - Charron MJ
AD  - Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology 
      and Infertility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 
      Bronx, NY, USA.
AD  - Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park 
      Avenue, Bronx, NY, USA.
AD  - Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park 
      Avenue, Bronx, NY, USA.
FAU - Buyuk, Erkan
AU  - Buyuk E
AD  - Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology 
      and Infertility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 
      Bronx, NY, USA.
LA  - eng
GR  - R01 NS045940/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160215
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (KISS1 protein, human)
RN  - 0 (KISS1R protein, human)
RN  - 0 (Kisspeptins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Kisspeptin-1)
RN  - 0 (Receptors, Peptide)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (anti-Mullerian hormone receptor)
RN  - 80497-65-0 (Anti-Mullerian Hormone)
SB  - IM
MH  - Aging/*genetics/pathology
MH  - Animals
MH  - Anti-Mullerian Hormone/biosynthesis/genetics
MH  - Chemokine CCL2/*genetics
MH  - Diet, High-Fat
MH  - Female
MH  - Fertilization in Vitro/methods
MH  - Gene Expression Regulation
MH  - Granulosa Cells/metabolism
MH  - Humans
MH  - Kisspeptins/*biosynthesis/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Obesity/*genetics/pathology
MH  - Ovary/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, G-Protein-Coupled/*biosynthesis/genetics
MH  - Receptors, Kisspeptin-1
MH  - Receptors, Peptide/biosynthesis/genetics
MH  - Receptors, Transforming Growth Factor beta/biosynthesis/genetics
PMC - PMC4818642
OTO - NOTNLM
OT  - Aging
OT  - Kisspeptin
OT  - MCP-1
OT  - Obesity
OT  - Ovary
EDAT- 2016/02/18 06:00
MHDA- 2016/12/17 06:00
PMCR- 2017/04/01
CRDT- 2016/02/17 06:00
PHST- 2015/11/25 00:00 [received]
PHST- 2016/01/26 00:00 [accepted]
PHST- 2016/02/17 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/12/17 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 10.1007/s10815-016-0672-x [pii]
AID - 672 [pii]
AID - 10.1007/s10815-016-0672-x [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2016 Apr;33(4):535-43. doi: 10.1007/s10815-016-0672-x. 
      Epub 2016 Feb 15.