PMID- 26879451
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1437
DP  - 2016 Mar 11
TI  - Analysis of endogenous aldehydes in human urine by static headspace gas 
      chromatography-mass spectrometry.
PG  - 241-246
LID - S0021-9673(16)30026-7 [pii]
LID - 10.1016/j.chroma.2016.01.056 [doi]
AB  - Endogenous aldehydes (EAs) generated during oxidative stress and cell processes 
      are associated with many pathogenic and toxicogenic processes. The aim of this 
      research was to develop a solvent-free and automated analytical method for the 
      determination of EAs in human urine using a static headspace generator sampler 
      coupled with gas chromatography-mass spectrometry (HS-GC-MS). Twelve significant 
      EAs used as markers of different biochemical and physiological processes, namely 
      short- and medium-chain alkanals, alpha,beta-unsaturated aldehydes and dicarbonyl 
      aldehydes have been selected as target analytes. Human urine samples (no dilution 
      is required) were derivatized with O-2,3,4,5,6-pentafluorobenzylhydroxylamine in 
      alkaline medium (hydrogen carbonate-carbonate buffer, pH 10.3). The HS-GC-MS 
      method developed renders an efficient tool for the sensitive and precise 
      determination of EAs in human urine with limits of detection from 1 to 15ng/L and 
      relative standard deviations, (RSDs) from 6.0 to 7.9%. Average recoveries by 
      enriching urine samples ranged between 92 and 95%. Aldehydes were readily 
      determined at 0.005-50mug/L levels in human urine from healthy subjects, smokers 
      and diabetic adults.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Serrano, Maria
AU  - Serrano M
AD  - Department of Analytical Chemistry, Campus of Rabanales, University of Cordoba, 
      E-14071 Cordoba, Spain.
FAU - Gallego, Mercedes
AU  - Gallego M
AD  - Department of Analytical Chemistry, Campus of Rabanales, University of Cordoba, 
      E-14071 Cordoba, Spain. Electronic address: qa1gafem@uco.es.
FAU - Silva, Manuel
AU  - Silva M
AD  - Department of Analytical Chemistry, Campus of Rabanales, University of Cordoba, 
      E-14071 Cordoba, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160203
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Aldehydes)
RN  - 0 (Solvents)
SB  - IM
MH  - Adult
MH  - Aldehydes/*analysis
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Sensitivity and Specificity
MH  - Solvents/analysis
MH  - Urinalysis/*instrumentation/*methods
OTO - NOTNLM
OT  - Endogenous aldehydes
OT  - Gas chromatography-mass spectrometry
OT  - Human urine
OT  - Oxidative stress
OT  - Static headspace technique
EDAT- 2016/02/18 06:00
MHDA- 2016/10/08 06:00
CRDT- 2016/02/17 06:00
PHST- 2015/11/24 00:00 [received]
PHST- 2016/01/15 00:00 [revised]
PHST- 2016/01/18 00:00 [accepted]
PHST- 2016/02/17 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - S0021-9673(16)30026-7 [pii]
AID - 10.1016/j.chroma.2016.01.056 [doi]
PST - ppublish
SO  - J Chromatogr A. 2016 Mar 11;1437:241-246. doi: 10.1016/j.chroma.2016.01.056. Epub 
      2016 Feb 3.