PMID- 26879576
OWN - NLM
STAT- MEDLINE
DCOM- 20160823
LR  - 20211103
IS  - 1471-2261 (Electronic)
IS  - 1471-2261 (Linking)
VI  - 16
DP  - 2016 Feb 16
TI  - Left ventricular deformation associated with cardiomyocyte Ca(2+) transients 
      delay in early stage of low-dose of STZ and high-fat diet induced type 2 diabetic 
      rats.
PG  - 41
LID - 10.1186/s12872-016-0220-8 [doi]
LID - 41
AB  - BACKGROUND: In the early stage of diabetes, the cardiac ejection fraction is 
      preserved, despite the existence of the subclinical cardiac dysfunction to some 
      extent. However, the detailed phenotype of this dysfunction and the underlying 
      mechanism remain unclear. To improve our understanding of this issue, we used 
      low-dose STZ and high-fat diet to induce type 2 diabetic models in rats. The 
      effects and the mechanism associated with the early stages of the disease were 
      analyzed. METHODS: The type 2 diabetic mellitus (T2DM) in SD rats were induced 
      through 30 mg/kg STZ and high-fat diet. Two-dimensional spackle-tracking 
      echocardiography (STE) and the dobutamine test were performed to examine the 
      cardiac function. Calcium transients of left ventricular myocytes were detected 
      and the related intracellular signalling factors were analyzed by western 
      blotting. RESULTS: After 6-weeks, T2DM rats in left ventricular (LV) diastole 
      showed decreased global and segment strain(S) levels (P < 0.05), both in the 
      radial and circumferential directions. Strain rate (Sr) abatement occurred in 
      three segments in the radial and circumferential directions (P < 0.05), and the 
      radial global Sr also decreased (P < 0.05). In the systolic LV, radial Sr was 
      reduced, except the segment of the anterior septum, and the Sr of the lateral 
      wall and post septum decreased in the circumferential direction (P < 0.05). 
      Conventional M-mode echocardiography failed to detect significant alterations of 
      cardiac performance between the two groups even after 12 weeks, and the decreased 
      ejection fraction (EF%), fractional shortening (FS%) and end-systolic diameters 
      (ESD) could be detected only under stress conditions induced by dobutamine (P < 
      0.05). In terms of calcium transients in cardiac myocytes, the Tpeak in model 
      rats at 6 weeks was not affected, while the Tdecay1/2 was higher than that of the 
      controls (P < 0.05), and both showed a dose-dependent delay after isoproterenol 
      treatment (P < 0.05). Western blot analysis showed that in 6-week T2DM rats, 
      myocardial p-PLB expression was elevated, whereas p-CaMKII, p-AMPK and Sirt1 were 
      significantly down-regulated (P < 0.05). CONCLUSION: A rat model of T2DM was 
      established by low dose STZ and a high-fat diet. LV deformation was observed in 
      the early stages of T2DM in association with the delay of Ca(2+) transients in 
      cardiomyocytes due to the decreased phosphorylation of CaMKII. Myocardial 
      metabolism remodeling might contribute to the early LV function and calcium 
      transportation abnormalities.
FAU - Liu, Xiao-Ying
AU  - Liu XY
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Liu, Fu-Cheng
AU  - Liu FC
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
AD  - Department of Cardiology of the First Affiliated Hospital, Jinan University, 
      Guangzhou, 510630, P.R. China.
FAU - Deng, Chun-Yu
AU  - Deng CY
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Zhang, Meng-Zhen
AU  - Zhang MZ
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Yang, Min
AU  - Yang M
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Xiao, Ding-Zhang
AU  - Xiao DZ
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Lin, Qiu-Xiong
AU  - Lin QX
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Cai, Shi-Ting
AU  - Cai ST
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Kuang, Su-Juan
AU  - Kuang SJ
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Chen, Jing
AU  - Chen J
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Chen, Shao-Xian
AU  - Chen SX
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Zhu, Jie-Ning
AU  - Zhu JN
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Yang, Hui
AU  - Yang H
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Rao, Fang
AU  - Rao F
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Fu, Yong-Heng
AU  - Fu YH
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China.
FAU - Yu, Xi-Yong
AU  - Yu XY
AD  - Guangdong Cardiovascular Institute and Medical Research Center, Guangdong General 
      Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Road, 
      Guangzhou, Guangdong, 510080, P.R. China. yuxycn@aliyun.com.
AD  - Institute of Molecular and Clinical Pharmacology, Guangzhou Medical University, 
      Guangzhou, 511436, P.R. China. yuxycn@aliyun.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160216
PL  - England
TA  - BMC Cardiovasc Disord
JT  - BMC cardiovascular disorders
JID - 100968539
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (phospholamban)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Calcium/*metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
MH  - Diabetes Mellitus, Experimental/complications/*metabolism
MH  - Diabetes Mellitus, Type 2/complications/*metabolism
MH  - Diabetic Cardiomyopathies/diagnostic imaging/etiology/*metabolism
MH  - *Diet, High-Fat
MH  - Disease Models, Animal
MH  - Echocardiography
MH  - Echocardiography, Stress
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Heart Ventricles/cytology/diagnostic imaging/*metabolism
MH  - Immunoblotting
MH  - Myocytes, Cardiac/*metabolism
MH  - Phosphoproteins/metabolism
MH  - Rats
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism
MH  - Sirtuin 1/metabolism
PMC - PMC4754853
EDAT- 2016/02/18 06:00
MHDA- 2016/08/24 06:00
PMCR- 2016/02/16
CRDT- 2016/02/17 06:00
PHST- 2015/07/20 00:00 [received]
PHST- 2016/02/09 00:00 [accepted]
PHST- 2016/02/17 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/08/24 06:00 [medline]
PHST- 2016/02/16 00:00 [pmc-release]
AID - 10.1186/s12872-016-0220-8 [pii]
AID - 220 [pii]
AID - 10.1186/s12872-016-0220-8 [doi]
PST - epublish
SO  - BMC Cardiovasc Disord. 2016 Feb 16;16:41. doi: 10.1186/s12872-016-0220-8.