PMID- 26880953
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160216
LR  - 20220311
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2016
DP  - 2016
TI  - Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates 
      Complex Promotes Skin Wound Healing by Paracrine Signaling.
PG  - 3269267
LID - 10.1155/2016/3269267 [doi]
LID - 3269267
AB  - Purpose. To probe growth characteristics of human umbilical cord mesenchymal stem 
      cells (hUCMSCs) cultured with alginate gel scaffolds, and to explore feasibility 
      of wound dressing model of hUCMSCs-alginates compound. Methods. hUCMSCs were 
      isolated, cultured, and identified in vitro. Then cells were cultivated in 100 mM 
      calcium alginate gel, and the capacity of proliferation and migration and the 
      expression of vascular endothelial growth factors (VEGF) were investigated 
      regularly. Wound dressing model of hUCMSCs-alginate gel mix was transplanted into 
      Balb/c mice skin defects. Wound healing rate and immunohistochemistry were 
      examined. Results. hUCMSCs grew well but with little migration ability in the 
      alginate gel. Compared with control group, a significantly larger cell number and 
      more VEGF expression were shown in the gel group after culturing for 3-6 days (P 
      < 0.05). In addition, a faster skin wound healing rate with more 
      neovascularization was observed in the hUCMSCs-alginate gel group than in control 
      groups at 15th day after surgery (P < 0.05). Conclusion. hUCMSCs can proliferate 
      well and express massive VEGF in calcium alginate gel porous scaffolds. Wound 
      dressing model of hUCMSCs-alginate gel mix can promote wound healing through 
      paracrine signaling.
FAU - Wang, Song
AU  - Wang S
AUID- ORCID: 0000-0002-6889-0412
AD  - Department of Vascular Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Yang, Huachao
AU  - Yang H
AD  - Department of Vascular Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Tang, Zhenrui
AU  - Tang Z
AD  - Department of Vascular Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Long, Gang
AU  - Long G
AD  - Department of Vascular Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Huang, Wen
AU  - Huang W
AD  - Department of Vascular Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
LA  - eng
PT  - Journal Article
DEP - 20151231
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4736208
EDAT- 2016/02/18 06:00
MHDA- 2016/02/18 06:01
PMCR- 2015/12/31
CRDT- 2016/02/17 06:00
PHST- 2015/04/11 00:00 [received]
PHST- 2015/09/14 00:00 [revised]
PHST- 2015/09/15 00:00 [accepted]
PHST- 2016/02/17 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/02/18 06:01 [medline]
PHST- 2015/12/31 00:00 [pmc-release]
AID - 10.1155/2016/3269267 [doi]
PST - ppublish
SO  - Stem Cells Int. 2016;2016:3269267. doi: 10.1155/2016/3269267. Epub 2015 Dec 31.