PMID- 26882313
OWN - NLM
STAT- MEDLINE
DCOM- 20160725
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - Development of a Xeno-Free Feeder-Layer System from Human Umbilical Cord 
      Mesenchymal Stem Cells for Prolonged Expansion of Human Induced Pluripotent Stem 
      Cells in Culture.
PG  - e0149023
LID - 10.1371/journal.pone.0149023 [doi]
LID - e0149023
AB  - Various feeder layers have been extensively applied to support the prolonged 
      growth of human pluripotent stem cells (hPSCs) for in vitro cultures. Among them, 
      mouse embryonic fibroblast (MEF) and mouse fibroblast cell line (SNL) are most 
      commonly used feeder cells for hPSCs culture. However, these feeder layers from 
      animal usually cause immunogenic contaminations, which compromises the potential 
      of hPSCs in clinical applications. In the present study, we tested human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) as a potent xeno-free feeder 
      system for maintaining human induced pluripotent stem cells (hiPSCs). The 
      hUC-MSCs showed characteristics of MSCs in xeno-free culture condition. On the 
      mitomycin-treated hUC-MSCs feeder, hiPSCs maintained the features of 
      undifferentiated human embryonic stem cells (hESCs), such as low efficiency of 
      spontaneous differentiation, stable expression of stemness markers, maintenance 
      of normal karyotypes, in vitro pluripotency and in vivo ability to form 
      teratomas, even after a prolonged culture of more than 30 passages. Our study 
      indicates that the xeno-free culture system may be a good candidate for growth 
      and expansion of hiPSCs as the stepping stone for stem cell research to further 
      develop better and safer stem cells.
FAU - Zou, Qing
AU  - Zou Q
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
AD  - Center for Stem Cell Research & Application, Institute of Blood Transfusion, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 
      Sichuan, China.
FAU - Wu, Mingjun
AU  - Wu M
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
FAU - Zhong, Liwu
AU  - Zhong L
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
FAU - Fan, Zhaoxin
AU  - Fan Z
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
FAU - Chen, Qiang
AU  - Chen Q
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
AD  - Center for Stem Cell Research & Application, Institute of Blood Transfusion, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 
      Sichuan, China.
FAU - Ma, Feng
AU  - Ma F
AD  - Research Center for Stem Cell and Regenerative Medicine, Sichuan Neo-life Stem 
      Cell Biotech INC., Chengdu, Sichuan, China.
AD  - Center for Stem Cell Research & Application, Institute of Blood Transfusion, 
      Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 
      Sichuan, China.
AD  - State Key Laboratory of Experimental Hematology, Chinese Academy of Medical 
      Sciences and Peking Union Medical College, Tianjin, China.
AD  - State Key Laboratory of Biotherapy, Collaborative Innovation Center for 
      Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160216
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MYC protein, human)
RN  - 0 (NANOG protein, human)
RN  - 0 (Nanog Homeobox Protein)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
RN  - 0 (ZFP42 protein, human)
RN  - 50SG953SK6 (Mitomycin)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/drug effects
MH  - Coculture Techniques
MH  - Embryonic Stem Cells/*cytology/metabolism
MH  - Feeder Cells/cytology/drug effects/metabolism
MH  - Fetal Blood/*cytology/drug effects/metabolism
MH  - Gene Expression
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology/drug effects/metabolism
MH  - Karyotype
MH  - Kruppel-Like Transcription Factors/genetics/metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Mitomycin/pharmacology
MH  - Nanog Homeobox Protein
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Octamer Transcription Factor-3/genetics/metabolism
MH  - Proto-Oncogene Proteins c-myc/genetics/metabolism
MH  - SOXB1 Transcription Factors/genetics/metabolism
MH  - Teratoma/pathology
PMC - PMC4755601
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/18 06:00
MHDA- 2016/07/28 06:00
PMCR- 2016/02/16
CRDT- 2016/02/17 06:00
PHST- 2015/11/04 00:00 [received]
PHST- 2016/01/26 00:00 [accepted]
PHST- 2016/02/17 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
PHST- 2016/02/16 00:00 [pmc-release]
AID - PONE-D-15-48334 [pii]
AID - 10.1371/journal.pone.0149023 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 16;11(2):e0149023. doi: 10.1371/journal.pone.0149023. 
      eCollection 2016.