PMID- 26883008
OWN - NLM
STAT- MEDLINE
DCOM- 20161226
LR  - 20161230
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 35
IP  - 4
DP  - 2016 Apr
TI  - MicroRNA-365a-3p promotes tumor growth and metastasis in laryngeal squamous cell 
      carcinoma.
PG  - 2017-26
LID - 10.3892/or.2016.4617 [doi]
AB  - MicroRNAs (miRNAs) are increasingly recognized as oncogenes or tumor suppressors 
      in laryngeal squamous cell carcinoma (LSCC). In this study, we analyzed the roles 
      of miR-365a-3p, miR-143-5p, and miR-494-3p in LSCC using Annexin V/propidium 
      iodide double staining and flow cyto-metry, along with a Transwell migration and 
      invasion assay. The results showed that miR-365a-3p inhibitor significantly 
      facilitated cell apoptosis and suppressed cell cycle progression, migration, and 
      invasion in Hep-2 cells. However, miR-143-5p and miR-494-3p had no such 
      influences. We then investigated the role of miR-365a-3p in LSCC in vivo and 
      found that miR-365a-3p inhibitor suppressed LSCC xenograft tumor growth and 
      metastasis in xenograft mouse models. Moreover, miR-365a-3p inhibitor 
      significantly decreased the expression of p-AKT (Ser473), which indicated that 
      miR-365a-3p can mediate PI3K/AKT signaling pathway transduction via p-AKT 
      (Ser473) in LSCC. The data suggest that miR-365a-3p may act as an oncomiR and may 
      promote growth and metastasis in LSCC via the PI3K/AKT signaling pathway, and 
      thus miR‑365a-3p may be a potential therapeutic target for treatment of LSCC.
FAU - Geng, Jiangqiao
AU  - Geng J
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Liu, Yuanhu
AU  - Liu Y
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Jin, Yaqiong
AU  - Jin Y
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Tai, Jun
AU  - Tai J
AD  - Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Xiao, Xiao
AU  - Xiao X
AD  - Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Chu, Ping
AU  - Chu P
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Yu, Yongbo
AU  - Yu Y
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Wang, Sheng Cai
AU  - Wang SC
AD  - Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Lu, Jie
AU  - Lu J
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Han, Shujing
AU  - Han S
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Shi, Jin
AU  - Shi J
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Guo, Yongli
AU  - Guo Y
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
FAU - Ni, Xin
AU  - Ni X
AD  - Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck 
      Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, 
      Capital Medical University, Beijing 100045, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160211
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (MIRN365 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - Hep G2 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Neoplasm Metastasis
MH  - Neoplasm Transplantation
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Signal Transduction
EDAT- 2016/02/18 06:00
MHDA- 2016/12/27 06:00
CRDT- 2016/02/18 06:00
PHST- 2015/10/09 00:00 [received]
PHST- 2015/11/10 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/12/27 06:00 [medline]
AID - 10.3892/or.2016.4617 [doi]
PST - ppublish
SO  - Oncol Rep. 2016 Apr;35(4):2017-26. doi: 10.3892/or.2016.4617. Epub 2016 Feb 11.