PMID- 26883668
OWN - NLM
STAT- MEDLINE
DCOM- 20170103
LR  - 20181113
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 243
IP  - 5
DP  - 2016 May
TI  - Characterization of wheat-Secale africanum chromosome 5R(a) derivatives carrying 
      Secale specific genes for grain hardness.
PG  - 1203-12
LID - 10.1007/s00425-016-2472-z [doi]
AB  - New wheat- Secale africanum chromosome 5R (a) substitution and translocation 
      lines were developed and identified by fluorescence in situ hybridization and 
      molecular markers, and chromosome 5R (a) specific genes responsible for grain 
      hardness were isolated. The wild species, Secale africanum Stapf. (genome 
      R(a)R(a)), serves as a valuable germplasm resource for increasing the diversity 
      of cultivated rye (S. cereale L., genome RR) and providing novel genes for wheat 
      improvement. In the current study, fluorescence in situ hybridization (FISH) and 
      molecular markers were applied to characterize new wheat-S. africanum chromosome 
      5R(a) derivatives. Labeled rye genomic DNA (GISH) and the Oligo-probes pSc119.2 
      and pTa535 (FISH) were used to study a wheat-S. africanum amphiploid and a 
      disomic 5R(a) (5D) substitution, and to identify a T5DL.5R(a)S translocation line 
      and 5R(a)S and 5R(a)L isotelosome lines. Twenty-one molecular markers were mapped 
      to chromosome 5R(a) arms which will facilitate future rapid identification of 
      5R(a) introgressions in wheat backgrounds. Comparative analysis of the molecular 
      markers mapped on 5R(a) with homoeologous regions in wheat confirmed a deletion 
      on the chromosome T5DL.5R(a)S, which suggests that the wheat-S. africanum 
      Robertsonian translocation involving homologous group 5 may not be fully 
      compensating. Complete coding sequences at the paralogous puroindoline-a (Pina) 
      and grain softness protein gene (Gsp-1) loci from S. africanum were cloned and 
      localized onto the short arm of chromosome 5R(a). The S. africanum chromosome 
      5R(a) substitution and translocation lines showed a reduction in the hardness 
      index, which may be associated with the S. africanum- specific Pina and Gsp-1 
      gene sequences. The present study reports the production of novel wheat-S. 
      africanum chromosome 5R(a) stripe rust resistant derivatives and new rye-specific 
      molecular markers, which may find application in future use of wild Secale genome 
      resources for grain quality studies and disease resistance breeding.
FAU - Li, Guangrong
AU  - Li G
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Gao, Dan
AU  - Gao D
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - La, Shixiao
AU  - La S
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Wang, Hongjin
AU  - Wang H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Li, Jianbo
AU  - Li J
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - He, Weilin
AU  - He W
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China.
FAU - Yang, Ennian
AU  - Yang E
AD  - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 
      610066, Sichuan, China.
FAU - Yang, Zujun
AU  - Yang Z
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, 610054, China. yangzujun@uestc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160216
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Basidiomycota/pathogenicity
MH  - *Chromosomes, Plant
MH  - Cloning, Molecular
MH  - Disease Resistance/genetics
MH  - Edible Grain/genetics
MH  - Genes, Plant
MH  - Genetic Markers
MH  - Hardness
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Diseases/genetics/microbiology
MH  - Secale/*genetics
MH  - Seeds/*physiology
MH  - Triticum/*genetics/microbiology
OTO - NOTNLM
OT  - Agronomic performance
OT  - Alien introgression
OT  - In situ hybridization
OT  - Secale africanum
EDAT- 2016/02/18 06:00
MHDA- 2017/01/04 06:00
CRDT- 2016/02/18 06:00
PHST- 2015/11/26 00:00 [received]
PHST- 2016/01/21 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2017/01/04 06:00 [medline]
AID - 10.1007/s00425-016-2472-z [pii]
AID - 10.1007/s00425-016-2472-z [doi]
PST - ppublish
SO  - Planta. 2016 May;243(5):1203-12. doi: 10.1007/s00425-016-2472-z. Epub 2016 Feb 
      16.