PMID- 26884487
OWN - NLM
STAT- MEDLINE
DCOM- 20170922
LR  - 20240109
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 171
IP  - 3
DP  - 2016 Jul
TI  - Singlet Oxygen-Induced Membrane Disruption and Serpin-Protease Balance in 
      Vacuolar-Driven Cell Death.
PG  - 1616-25
LID - 10.1104/pp.15.02026 [doi]
AB  - Singlet oxygen plays a role in cellular stress either by providing direct 
      toxicity or through signaling to initiate death programs. It was therefore of 
      interest to examine cell death, as occurs in Arabidopsis, due to differentially 
      localized singlet oxygen photosensitizers. The photosensitizers rose bengal (RB) 
      and acridine orange (AO) were localized to the plasmalemma and vacuole, 
      respectively. Their photoactivation led to cell death as measured by ion leakage. 
      Cell death could be inhibited by the singlet oxygen scavenger histidine in 
      treatments with AO but not with RB In the case of AO treatment, the vacuolar 
      membrane was observed to disintegrate. Concomitantly, a complex was formed 
      between a vacuolar cell-death protease, RESPONSIVE TO DESSICATION-21 and its 
      cognate cytoplasmic protease inhibitor ATSERPIN1. In the case of RB treatment, 
      the tonoplast remained intact and no complex was formed. Over-expression of 
      AtSerpin1 repressed cell death, only under AO photodynamic treatment. 
      Interestingly, acute water stress showed accumulation of singlet oxygen as 
      determined by fluorescence of Singlet Oxygen Sensor Green, by electron 
      paramagnetic resonance spectroscopy and the induction of singlet oxygen marker 
      genes. Cell death by acute water stress was inhibited by the singlet oxygen 
      scavenger histidine and was accompanied by vacuolar collapse and the appearance 
      of serpin-protease complex. Over-expression of AtSerpin1 also attenuated cell 
      death under this mode of cell stress. Thus, acute water stress damage shows 
      parallels to vacuole-mediated cell death where the generation of singlet oxygen 
      may play a role.
CI  - (c) 2016 American Society of Plant Biologists. All Rights Reserved.
FAU - Koh, Eugene
AU  - Koh E
AUID- ORCID: 0000-0003-1073-0050
AD  - Department of Plant and Environmental Sciences, Weizmann Institute, Rehovot, 
      Israel (E.K., A.M., R.F.); and Department of Chemical Research Support, Weizmann 
      Institute, Rehovot, Israel (R.C.).
FAU - Carmieli, Raanan
AU  - Carmieli R
AD  - Department of Plant and Environmental Sciences, Weizmann Institute, Rehovot, 
      Israel (E.K., A.M., R.F.); and Department of Chemical Research Support, Weizmann 
      Institute, Rehovot, Israel (R.C.).
FAU - Mor, Avishai
AU  - Mor A
AD  - Department of Plant and Environmental Sciences, Weizmann Institute, Rehovot, 
      Israel (E.K., A.M., R.F.); and Department of Chemical Research Support, Weizmann 
      Institute, Rehovot, Israel (R.C.).
FAU - Fluhr, Robert
AU  - Fluhr R
AD  - Department of Plant and Environmental Sciences, Weizmann Institute, Rehovot, 
      Israel (E.K., A.M., R.F.); and Department of Chemical Research Support, Weizmann 
      Institute, Rehovot, Israel (R.C.) robert.fluhr@weizmann.ac.il.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160216
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Serpin1 protein, Arabidopsis)
RN  - 0 (Serpins)
RN  - 17778-80-2 (Singlet Oxygen)
RN  - 1ZPG1ELY14 (Rose Bengal)
RN  - EC 3.4.- (Cysteine Proteases)
RN  - EC 3.4.- (RD21 protein, Arabidopsis)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Arabidopsis/*cytology/drug effects/genetics/metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Cell Death/drug effects
MH  - Cysteine Proteases/genetics/metabolism
MH  - Cytoplasm/metabolism
MH  - Darkness
MH  - Gene Expression Regulation, Plant
MH  - Plant Cells/drug effects/metabolism
MH  - Plants, Genetically Modified
MH  - Rose Bengal/pharmacology
MH  - Serpins/genetics/*metabolism
MH  - Singlet Oxygen/*metabolism
MH  - Vacuoles/drug effects/*metabolism
PMC - PMC4936544
EDAT- 2016/02/18 06:00
MHDA- 2017/09/25 06:00
PMCR- 2016/02/16
CRDT- 2016/02/18 06:00
PHST- 2016/01/04 00:00 [received]
PHST- 2016/02/12 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2017/09/25 06:00 [medline]
PHST- 2016/02/16 00:00 [pmc-release]
AID - pp.15.02026 [pii]
AID - PP201502026DR1 [pii]
AID - 10.1104/pp.15.02026 [doi]
PST - ppublish
SO  - Plant Physiol. 2016 Jul;171(3):1616-25. doi: 10.1104/pp.15.02026. Epub 2016 Feb 
      16.