PMID- 26884848
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20190221
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 12
DP  - 2015
TI  - Full-length spleen tyrosine kinase inhibits the invasion and metastasis of human 
      laryngeal squamous cell carcinoma.
PG  - 15786-93
AB  - OBJECTIVE: This study aimed to investigate correlation between full-length spleen 
      tyrosine kinase [SYK (L)] expression and clinical characteristics of laryngeal 
      squamous cell carcinoma (LSCC), and explore effects of SYK (L) on invasion and 
      metastasis of LSCC. METHODS: The human laryngeal cancer Hep-2 cells with low SYK 
      (L) expression were transfected with pIRES2-EGFP-SYK (L) vector and empty vector 
      pIRES2-EGFP to generate Hep-2-SYK (L) cells and Hep-2-neo cells. The cell 
      invasion and migration abilities were determined. RESULTS: The SYK (L) positive 
      expression rate in LSCC tissues was significantly lower than in vocal cord 
      dysplasia tissues and normal laryngeal tissues (P < 0.05). There was a 
      significant correlation between SYK (L) expression and LSCC T stage, 
      histopathological grade and lymph node metastasis (P < 0.05). mRNA expression of 
      SYK (L) in Hep-2-SYK (L) cells was significantly higher than in Hep-2-neo cells 
      and Hep-2 cells (P < 0.01). The protein expression of SYK (L) in Hep-2-SYK (L) 
      cells was markedly higher than in Hep-2-neo cells and Hep-2 cells (P < 0.01). The 
      number of invasive cells was significantly lower in Hep-2-SYK (L) group than in 
      Hep-2-neo group and Hep-2 group (P < 0.01). The average number of migrating cells 
      in Hep-2-SYK (L) group also markedly reduced as compared to Hep-2-neo group and 
      Hep-2 group (P < 0.01). CONCLUSION: The SYK (L) expression was down-regulated in 
      LSCC, which was closely correlated with cancer growth and lymph node metastasis. 
      SYK (L) up-regulation was able to inhibit the invasion and metastasis of LSCC, 
      therefore suppressing tumor development. Thus, SYK (L) may be a potential target 
      for the LSCC treatment.
FAU - Li, Zhihai
AU  - Li Z
AD  - Department of Otolaryngology, Taizhou Municipal Hospital Taizhou 318000, 
      Zhejiang, China.
FAU - Cai, Zhiyi
AU  - Cai Z
AD  - Department of Otolaryngology, Taizhou Municipal HospitalTaizhou 318000, Zhejiang, 
      China; The First Clinical College of Wenzhou Medical UniversityWhenzhou, China.
FAU - Tao, Baohong
AU  - Tao B
AD  - Department of Otolaryngology, Taizhou Municipal Hospital Taizhou 318000, 
      Zhejiang, China.
FAU - Jin, Qiaozhi
AU  - Jin Q
AD  - Department of Otolaryngology, Taizhou Municipal Hospital Taizhou 318000, 
      Zhejiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151201
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (SYK protein, human)
RN  - EC 2.7.10.2 (Syk Kinase)
SB  - IM
MH  - Carcinoma, Squamous Cell/*enzymology/genetics/secondary
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*enzymology/genetics/pathology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Laryngeal Neoplasms/*enzymology/genetics/pathology
MH  - Lymphatic Metastasis
MH  - Neoplasm Grading
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Protein-Tyrosine Kinases/genetics/*metabolism
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Syk Kinase
MH  - Transfection
PMC - PMC4730061
OTO - NOTNLM
OT  - Full-length spleen tyrosine kinase
OT  - invasion
OT  - laryngeal cancer
OT  - metastasis
EDAT- 2016/02/18 06:00
MHDA- 2016/11/01 06:00
PMCR- 2015/12/01
CRDT- 2016/02/18 06:00
PHST- 2015/10/15 00:00 [received]
PHST- 2015/11/28 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Dec 1;8(12):15786-93. eCollection 2015.