PMID- 26884859
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20181113
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 12
DP  - 2015
TI  - IVIG inhibits TNF-alpha-induced MMP9 expression and activity in monocytes by 
      suppressing NF-kappaB and P38 MAPK activation.
PG  - 15879-86
AB  - Matrix metalloproteinase-9 (MMP9) has been involved in inflammatory and 
      pathologic processes of coronary artery lesions (CAL) in Kawasaki disease (KD). 
      Intravenous immunoglobulin (IVIG), a traditional treatment for Kawasaki disease, 
      could decrease the expressions of MMP9. The purpose of this study was to 
      investigate the protective effect of IVIG in chemotactic migration of monocyte 
      and the regulation of MMP9 induced by tumor necrosis factor-alpha (TNF-alpha) in U937s. 
      Studies were carried out with real time polymerase chain reaction (RT-PCR), 
      zymographic, Western blotting and immunofluorescence. U937s' migration was 
      enhanced by TNF-alpha stimulation, while was inhibited by IVIG pretreatment. MMP9 
      expression and activity in U937s were also significantly enhanced by TNF-alpha and 
      inhibited by IVIVG pretreatment. During inflammatory stimulus, nuclear factor 
      kappa B (NF-kappaB) and P38 Mitogenactivated protein kinase (P38 MAPK) pathways play 
      a significant role in regulating MMP9 gene expression. TNF-alpha induced nuclear 
      translocation of NF-kappaB and P38 MAPK activation in U937s were inhibited 
      significantly by IVIG. Furthermore, we clarified that nuclear NF-kappaB and P38 MAPK 
      pathways play pivotal roles in regulating U937s' migration and MMP9 expressions 
      using PDTC and SB203580, which were specific inhibitors of NF-kappaB and p38 MAPK 
      pathways. IVIG displays striking biological effects, notably promoting monocyte 
      migration. These effects involve the NF-kappaB and p38 pathways, and increased MMP9 
      activity. It might be a crucial mechanism of IVIG reducing the occurrence of CAL 
      that IVIG inhibited monocytes expressing MMP9 and decreased chemotactic migration 
      of monocyte.
FAU - Zhou, Cuizhen
AU  - Zhou C
AD  - Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong 
      University Shanghai 200062, China.
FAU - Huang, Min
AU  - Huang M
AD  - Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong 
      University Shanghai 200062, China.
FAU - Xie, Lijian
AU  - Xie L
AD  - Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong 
      University Shanghai 200062, China.
FAU - Shen, Jie
AU  - Shen J
AD  - Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong 
      University Shanghai 200062, China.
FAU - Xiao, Tingting
AU  - Xiao T
AD  - Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong 
      University Shanghai 200062, China.
FAU - Wang, Renjian
AU  - Wang R
AD  - Department of Cardiology, Shanghai Children's Hospital, Shanghai Jiaotong 
      University Shanghai 200062, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151201
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (NF-kappa B)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Chemotaxis/drug effects
MH  - Enzyme Activation
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Immunoglobulins, Intravenous/*pharmacology
MH  - Matrix Metalloproteinase 9/genetics/*metabolism
MH  - Monocytes/*drug effects/enzymology
MH  - NF-kappa B/antagonists & inhibitors/*metabolism
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - U937 Cells
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
PMC - PMC4730072
OTO - NOTNLM
OT  - IVIG
OT  - MMP9
OT  - NF-kappaB
OT  - P38 MAPK
OT  - monocyte migration
EDAT- 2016/02/18 06:00
MHDA- 2016/11/01 06:00
PMCR- 2015/12/01
CRDT- 2016/02/18 06:00
PHST- 2015/10/02 00:00 [received]
PHST- 2015/11/25 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Dec 1;8(12):15879-86. eCollection 2015.