PMID- 26885021
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160217
LR  - 20200930
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 11
DP  - 2015
TI  - Experimental studies on islets isolation, purification and function in rats.
PG  - 20932-8
AB  - To develop a simple and effective method of islet isolation and purification in 
      rats. Collagenase P was injected into pancreatic duct followed by incubation in 
      water bath to digest the pancreas and isolate islet, then discontinuous gravity 
      gradient purification was used to purify the islet. The purified islets were 
      identified by dithizone staining. The viability of islets was assessed by 
      fluorescence staining of acridine orange (AO) and propidium iodide (PI). The 
      function of purified islets was determined by glucose-stimulated insulin release 
      test and transplantation of rat with streptozocin-induced diabetes. 738+/-193 
      islets were recovered after purification. The average purity was 77+/-13%, the 
      viability of islets was more than 95%. When inspected by glucose stimulation, the 
      secreted insulin concentration was 24.31+/-5.47 mIU/L when stimulated by low 
      concentration glucose and 37.62+/-4.29 mIU/L by high concentration glucose. There 
      was significant difference between the two phases (P<0.05). The blood sugar 
      concentration recovered to normal level after two days in the animals with islet 
      transplantation. In conclusion, islets can be procured with good function and 
      shape by using the method of injecting collagenase into pancreatic duct followed 
      by incubation in water bath and purification using discontinuous gravity 
      gradient.
FAU - Pang, Xinlu
AU  - Pang X
AD  - Department of Kidney Transplantation, The First Affiliated Hospital of Zhengzhou 
      University Zhengzhou 450052, China.
FAU - Xue, Wujun
AU  - Xue W
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - Feng, Xinshun
AU  - Feng X
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - Tian, Xiaohui
AU  - Tian X
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - Teng, Yan
AU  - Teng Y
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - Ding, Xiaoming
AU  - Ding X
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - Pan, Xiaoming
AU  - Pan X
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - Guo, Qi
AU  - Guo Q
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
FAU - He, Xiaoli
AU  - He X
AD  - Hospital of Nephrology, The First Affiliated Hospital of Medical College, Xi'an 
      Jiaotong University Xi'an 710061, China.
LA  - eng
PT  - Journal Article
DEP - 20151115
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4723866
OTO - NOTNLM
OT  - Islet
OT  - isolation
OT  - purification
EDAT- 2016/02/18 06:00
MHDA- 2016/02/18 06:01
PMCR- 2015/11/15
CRDT- 2016/02/18 06:00
PHST- 2015/09/04 00:00 [received]
PHST- 2015/11/02 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/02/18 06:01 [medline]
PHST- 2015/11/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Nov 15;8(11):20932-8. eCollection 2015.