PMID- 26885862
OWN - NLM
STAT- MEDLINE
DCOM- 20160630
LR  - 20211203
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 44
IP  - 2
DP  - 2016 Feb 16
TI  - Analysis of the Rab GTPase Interactome in Dendritic Cells Reveals Anti-microbial 
      Functions of the Rab32 Complex in Bacterial Containment.
PG  - 422-37
LID - S1074-7613(16)30014-0 [pii]
LID - 10.1016/j.immuni.2016.01.027 [doi]
AB  - Dendritic cells (DCs) orchestrate complex membrane trafficking through an 
      interconnected transportation network linked together by Rab GTPases. Through 
      a tandem affinity purification strategy and mass spectrometry, we depicted an 
      interactomic landscape of major members of the mammalian Rab GTPase family. When 
      complemented with imaging tools, this proteomic analysis provided a global view 
      of intracellular membrane organization. Driven by this analysis, we investigated 
      dynamic changes to the Rab32 subnetwork in DCs induced by L. monocytogenes 
      infection and uncovered an essential role of this subnetwork in controlling the 
      intracellular proliferation of L. monocytogenes. Mechanistically, Rab32 formed a 
      persistent complex with two interacting proteins, PHB and PHB2, to encompass 
      bacteria both during early phagosome formation and after L. monocytogenes escaped 
      the original containment vacuole. Collectively, we have provided a functional 
      compartmentalization overview and an organizational framework of intracellular 
      Rab-mediated vesicle trafficking that can serve as a resource for future 
      investigations.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Li, Yuanyuan
AU  - Li Y
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Zou, Liyun
AU  - Zou L
AD  - Department of Immunology, Third Military Medical University, Chongqing 400038, 
      China.
FAU - Tang, Xiangyu
AU  - Tang X
AD  - Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin 
      300000 China.
FAU - Yang, Yi
AU  - Yang Y
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Ma, Li
AU  - Ma L
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Jia, Qingzhu
AU  - Jia Q
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Ni, Qingshan
AU  - Ni Q
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Liu, Siqi
AU  - Liu S
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Tang, Lizhang
AU  - Tang L
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Lin, Regina
AU  - Lin R
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Wong, Elizabeth
AU  - Wong E
AD  - Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
FAU - Sun, Wei
AU  - Sun W
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Wang, Liting
AU  - Wang L
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Wei, Quanfang
AU  - Wei Q
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Ran, Haiying
AU  - Ran H
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Zhang, Liqun
AU  - Zhang L
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Lian, Hengning
AU  - Lian H
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Huang, Wei
AU  - Huang W
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
FAU - Wu, Yuzhang
AU  - Wu Y
AD  - Department of Immunology, Third Military Medical University, Chongqing 400038, 
      China.
FAU - Li, Qi-Jing
AU  - Li QJ
AD  - Chongqing Key Laboratory of Cytomics, Chongqing 400038, China; Department of 
      Immunology, Duke University Medical Center, Durham, NC 27710, USA. Electronic 
      address: qi-jing.li@duke.edu.
FAU - Wan, Ying
AU  - Wan Y
AD  - Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, 
      China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China. Electronic 
      address: wanying.cn@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Phb2 protein, mouse)
RN  - 0 (Prohibitins)
RN  - 0 (Repressor Proteins)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (poly-beta-hydroxybutyrate polymerase)
RN  - EC 3.6.1.- (Rab32 protein, mouse)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - Acyltransferases/metabolism
MH  - Animals
MH  - Anti-Infective Agents/therapeutic use
MH  - Cell Line
MH  - Computational Biology
MH  - Containment of Biohazards
MH  - Dendritic Cells/*immunology/microbiology
MH  - Listeria monocytogenes/growth & development/*immunology
MH  - Listeriosis/drug therapy/*immunology
MH  - Mice
MH  - Multiprotein Complexes/*metabolism
MH  - Prohibitins
MH  - Protein Transport
MH  - Repressor Proteins/metabolism
MH  - Vacuoles/metabolism
MH  - rab GTP-Binding Proteins/*metabolism
EDAT- 2016/02/18 06:00
MHDA- 2016/07/01 06:00
CRDT- 2016/02/18 06:00
PHST- 2015/07/07 00:00 [received]
PHST- 2015/10/28 00:00 [revised]
PHST- 2015/11/17 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/07/01 06:00 [medline]
AID - S1074-7613(16)30014-0 [pii]
AID - 10.1016/j.immuni.2016.01.027 [doi]
PST - ppublish
SO  - Immunity. 2016 Feb 16;44(2):422-37. doi: 10.1016/j.immuni.2016.01.027.