PMID- 26886067 OWN - NLM STAT- MEDLINE DCOM- 20161027 LR - 20161230 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 64 IP - 9 DP - 2016 Mar 9 TI - Purification and Characterization of Protamine, the Allergen from the Milt of Large Yellow Croaker (Pseudosciaena crocea), and Its Components. PG - 1999-2011 LID - 10.1021/acs.jafc.5b05899 [doi] AB - The protamine in fish milt can cause anaphylaxis in humans. To determine the allergen in the milt of large yellow croaker (Pseudosciaena crocea), crude extracts were incubated with sera from allergic patients. The results showed that a 12 kDa multicomponent protein was the major allergen in the milt of large yellow croaker. The multicomponent protein was purified, and physicochemical characterization showed that it was a glycoprotein, highly stable in acid-alkali conditions, and weakly retained immunoglobulin E (IgE)-binding activity at high temperatures. Separation and immunoreactivity analysis of the components of the multicomponent protein showed that it had six components, and component 5 had the strongest IgE-binding activity with patient sera. N-terminal sequencing confirmed the multicomponent protein was protamine. Following analysis of protamine from different fish by reversed-phase liquid chromatography and circular dichroism spectra, the protamines from different fish were found to have a similar secondary structure, although their components were different. FAU - Liu, Yan-Yun AU - Liu YY AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. FAU - Chen, Xiao-Feng AU - Chen XF AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. FAU - Hu, Jia-Wei AU - Hu JW AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. FAU - Chen, Zhong-Wei AU - Chen ZW AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. FAU - Zhang, Ling-Jing AU - Zhang LJ AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. FAU - Cao, Min-Jie AU - Cao MJ AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. FAU - Liu, Guang-Ming AU - Liu GM AD - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University , 43 Yindou Road, Xiamen, Fujian 361021, China. AD - Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , Xiamen. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160226 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Allergens) RN - 0 (Amino Acids) RN - 0 (Fish Proteins) RN - 0 (Glycoproteins) RN - 0 (Nucleoproteins) RN - 0 (Protamines) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Adult MH - Allergens/chemistry/immunology/*isolation & purification MH - Amino Acid Sequence MH - Amino Acids/analysis MH - Animals MH - China MH - Chromatography, High Pressure Liquid MH - Female MH - Fish Proteins/chemistry/*immunology/isolation & purification MH - Food Hypersensitivity/immunology MH - Glycoproteins/analysis MH - Humans MH - Immunoglobulin E/metabolism MH - Male MH - Molecular Sequence Data MH - Nucleoproteins MH - Perciformes/*immunology MH - Protamines/*immunology/*isolation & purification MH - Protein Structure, Secondary MH - Semen/*immunology MH - Sequence Analysis, Protein MH - Young Adult OTO - NOTNLM OT - allergen OT - characterization OT - components OT - large yellow croaker (Pseudosciaena crocea) OT - milt OT - protamine EDAT- 2016/02/18 06:00 MHDA- 2016/11/01 06:00 CRDT- 2016/02/18 06:00 PHST- 2016/02/18 06:00 [entrez] PHST- 2016/02/18 06:00 [pubmed] PHST- 2016/11/01 06:00 [medline] AID - 10.1021/acs.jafc.5b05899 [doi] PST - ppublish SO - J Agric Food Chem. 2016 Mar 9;64(9):1999-2011. doi: 10.1021/acs.jafc.5b05899. Epub 2016 Feb 26.