PMID- 26886282
OWN - NLM
STAT- MEDLINE
DCOM- 20170209
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 8
DP  - 2016 Aug
TI  - Identification of proteins derived from Listeria monocytogenes inducing human 
      dendritic cell maturation.
PG  - 10893-907
LID - 10.1007/s13277-016-4933-1 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that can promote 
      antitumor immunity when pulsed with tumor antigens and then matured by 
      stimulatory agents. Despite apparent progress in DC-based cancer immunotherapy, 
      some discrepancies were reported in generating potent DCs. Listeria monocytogenes 
      as an intracellular microorganism is able to effectively activate DCs through 
      engaging pattern-recognition receptors (PRRs). This study aimed to find the most 
      potent components derived from L. monocytogenes inducing DC maturation. The 
      preliminary results demonstrated that the ability of protein components is higher 
      than DNA components to promote DC maturation and activation. Protein lysate 
      fractionation demonstrated that fraction 2 HIC (obtained by hydrophobic 
      interaction chromatography) was able to efficiently mature DCs. F2HIC-matured DCs 
      are able to induce allogeneic CD8(+) T cells proliferation better than 
      LPS-matured DCs and induce IFN-gamma producing CD8(+) T cells. Mass spectrometry 
      results showed that F2HIC contains 109 proteins. Based on the bioinformatics 
      analysis for these 109 proteins, elongation factor Tu (EF-Tu) could be considered 
      as a PRR ligand for stimulating DC maturation.
FAU - Mirzaei, Reza
AU  - Mirzaei R
AD  - Immunology Department, School of Medicine, Tehran University of Medical Sciences, 
      Poursina Avenue, Tehran, Iran.
FAU - Saei, Azad
AU  - Saei A
AD  - Immunology Department, School of Medicine, Tehran University of Medical Sciences, 
      Poursina Avenue, Tehran, Iran.
FAU - Torkashvand, Fatemeh
AU  - Torkashvand F
AD  - Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, 
      Tehran, Iran.
FAU - Azarian, Bahareh
AU  - Azarian B
AD  - Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, 
      Tehran, Iran.
FAU - Jalili, Ahmad
AU  - Jalili A
AD  - Division of Immunology, Allergy and Infectious Diseases, Department of 
      Dermatology, Medical University of Vienna, Vienna, Austria.
FAU - Noorbakhsh, Farshid
AU  - Noorbakhsh F
AD  - Immunology Department, School of Medicine, Tehran University of Medical Sciences, 
      Poursina Avenue, Tehran, Iran.
FAU - Vaziri, Behrouz
AU  - Vaziri B
AD  - Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, 
      Tehran, Iran.
FAU - Hadjati, Jamshid
AU  - Hadjati J
AD  - Immunology Department, School of Medicine, Tehran University of Medical Sciences, 
      Poursina Avenue, Tehran, Iran. hajatij@sina.tums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20160217
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Bacterial Proteins)
RN  - 0 (Receptors, Pattern Recognition)
RN  - EC 3.6.1.- (Peptide Elongation Factor Tu)
SB  - IM
MH  - Bacterial Proteins/immunology
MH  - Cell Differentiation/*immunology
MH  - Cell Line
MH  - Dendritic Cells/cytology/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunotherapy/methods
MH  - Listeria monocytogenes/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Peptide Elongation Factor Tu/*immunology
MH  - Receptors, Pattern Recognition/immunology
OTO - NOTNLM
OT  - Dendritic cell
OT  - Elongation factor Tu
OT  - Listeria monocytogenes
OT  - Tumor
EDAT- 2016/02/18 06:00
MHDA- 2017/02/10 06:00
CRDT- 2016/02/18 06:00
PHST- 2015/11/26 00:00 [received]
PHST- 2016/01/28 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2017/02/10 06:00 [medline]
AID - 10.1007/s13277-016-4933-1 [pii]
AID - 10.1007/s13277-016-4933-1 [doi]
PST - ppublish
SO  - Tumour Biol. 2016 Aug;37(8):10893-907. doi: 10.1007/s13277-016-4933-1. Epub 2016 
      Feb 17.