PMID- 26886559
OWN - NLM
STAT- MEDLINE
DCOM- 20160802
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - Tissue Specific Expression of Cre in Rat Tyrosine Hydroxylase and Dopamine Active 
      Transporter-Positive Neurons.
PG  - e0149379
LID - 10.1371/journal.pone.0149379 [doi]
LID - e0149379
AB  - The rat is a preferred model system over the mouse for neurological studies, and 
      cell type-specific Cre expression in the rat enables precise ablation of gene 
      function in neurons of interest, which is especially valuable for 
      neurodegenerative disease modeling and optogenetics. Yet, few such Cre rats are 
      available. Here we report the characterization of two Cre rats, tyrosine 
      hydroxylase (TH)-Cre and dopamine active transporter (DAT or Slc6a3)-Cre, by 
      using a combination of immunohistochemistry (IHC) and mRNA fluorescence in situ 
      hybridization (FISH) as well as a fluorescent reporter for Cre activity. We 
      detected Cre expression in expected neurons in both Cre lines. Interestingly, we 
      also found that in Th-Cre rats, but not DAT-Cre rats, Cre is expressed in female 
      germ cells, allowing germline excision of the floxed allele and hence the 
      generation of whole-body knockout rats. In summary, our data demonstrate that 
      targeted integration of Cre cassette lead to faithful recapitulation of 
      expression pattern of the endogenous promoter, and mRNA FISH, in addition to IHC, 
      is an effective method for the analysis of the spatiotemporal gene expression 
      patterns in the rat brain, alleviating the dependence on high quality antibodies 
      that are often not available against rat proteins. The Th-Cre and the DAT-Cre rat 
      lines express Cre in selective subsets of dopaminergic neurons and should be 
      particularly useful for researches on Parkinson's disease.
FAU - Liu, Zhenyi
AU  - Liu Z
AD  - SAGE Labs, A Horizon Discovery Group Company, Saint Louis, MO, United States of 
      America.
FAU - Brown, Andrew
AU  - Brown A
AD  - SAGE Labs, A Horizon Discovery Group Company, Saint Louis, MO, United States of 
      America.
FAU - Fisher, Dan
AU  - Fisher D
AD  - SAGE Labs, A Horizon Discovery Group Company, Saint Louis, MO, United States of 
      America.
FAU - Wu, Yumei
AU  - Wu Y
AD  - SAGE Labs, A Horizon Discovery Group Company, Saint Louis, MO, United States of 
      America.
FAU - Warren, Joe
AU  - Warren J
AD  - SAGE Labs, A Horizon Discovery Group Company, Saint Louis, MO, United States of 
      America.
FAU - Cui, Xiaoxia
AU  - Cui X
AD  - SAGE Labs, A Horizon Discovery Group Company, Saint Louis, MO, United States of 
      America.
LA  - eng
PT  - Journal Article
DEP - 20160217
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
EIN - PLoS One. 2019 Jun 20;14(6):e0218976. PMID: 31220192
MH  - Alleles
MH  - Animals
MH  - Brain/*metabolism
MH  - Dopamine Plasma Membrane Transport Proteins/*metabolism
MH  - Dopaminergic Neurons/*metabolism
MH  - Embryo, Mammalian/metabolism
MH  - Germ Cells/metabolism
MH  - Humans
MH  - Integrases/*metabolism
MH  - Meiosis
MH  - Mutagenesis, Insertional
MH  - Oocytes/metabolism
MH  - *Organ Specificity
MH  - Rats
MH  - Substantia Nigra/metabolism
MH  - Tyrosine 3-Monooxygenase/genetics/*metabolism
MH  - Ventral Tegmental Area/metabolism
PMC - PMC4757100
COIS- Competing Interests: All authors are full time employees of Horizon Discovery 
      Group Company, which creates and markets genetically engineered rodent and rabbit 
      models, including these Cre rats. This does not alter the authors' adherence to 
      PLOS ONE policies on sharing data and materials.
EDAT- 2016/02/18 06:00
MHDA- 2016/08/03 06:00
PMCR- 2016/02/17
CRDT- 2016/02/18 06:00
PHST- 2015/05/07 00:00 [received]
PHST- 2016/02/01 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/08/03 06:00 [medline]
PHST- 2016/02/17 00:00 [pmc-release]
AID - PONE-D-15-16891 [pii]
AID - 10.1371/journal.pone.0149379 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 17;11(2):e0149379. doi: 10.1371/journal.pone.0149379. 
      eCollection 2016.