PMID- 26886741
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 12
DP  - 2016 May
TI  - TIRF high-content assay development for the evaluation of drug efficacy of 
      chemotherapeutic agents against EGFR-/HER2-positive breast cancer cell lines.
PG  - 3233-8
LID - 10.1007/s00216-016-9387-1 [doi]
AB  - Elevated expression of epidermal growth factor receptor (EGFR) is reported to be 
      associated with poor prognosis in breast cancer. EGFR subtype identification 
      plays a crucial role in deciding the drug combination to treat the cancer 
      patients. Conventional application of immunohistochemistry (IHC) and fluorescence 
      in situ hybridization (FISH) produces more discordance results in EGFR subtype 
      identification of cancer specimens. The present study is designed to develop an 
      analytical method for simultaneous identification of cell surface biomarkers and 
      quantitative estimation of drug efficacy in cancer specimens. For this study, we 
      have utilized a total internal reflection fluorescence microscope (TIRFM), Qdot 
      molecular probes and chemotherapeutic agent camptothecin (CPT)-treated breast 
      cancer cell lines namely MCF-7, SK-BR-3 and JIMT-1. Highly sensitive detection 
      signals with low background noise generated from the evanescent field excitation 
      of TIRFM make it a highly suitable tool to detect the cell surface biomarkers in 
      living cells. Moreover, single wavelength excitation of Qdot probes offers 
      multicolour imaging with strong emission brightness. In the present study, TIRF 
      high-content imaging system simultaneously showed the expression pattern of EGFRs 
      and EC50 value for CPT-induced apoptosis and necrosis in MCF-7, SK-BR-3 and 
      JIMT-1 cancer cell lines.
FAU - Ki, Jieun
AU  - Ki J
AD  - College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-ku, Seoul, 
      151-742, Republic of Korea.
FAU - Arumugam, Parthasarathy
AU  - Arumugam P
AD  - College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-ku, Seoul, 
      151-742, Republic of Korea.
FAU - Song, Joon Myong
AU  - Song JM
AD  - College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-ku, Seoul, 
      151-742, Republic of Korea. jmsong@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160217
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Antineoplastic Agents)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Antineoplastic Agents/*therapeutic use
MH  - Breast Neoplasms/*drug therapy/metabolism/pathology
MH  - Cell Line, Tumor
MH  - ErbB Receptors/*metabolism
MH  - Female
MH  - *Genes, erbB-2
MH  - Humans
OTO - NOTNLM
OT  - Camptothecin
OT  - Epidermal growth factor receptor
OT  - Human epidermal receptor-2
OT  - Total internal reflection fluorescence microscope
EDAT- 2016/02/18 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/18 06:00
PHST- 2015/11/24 00:00 [received]
PHST- 2016/02/02 00:00 [accepted]
PHST- 2016/02/18 06:00 [entrez]
PHST- 2016/02/18 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1007/s00216-016-9387-1 [pii]
AID - 10.1007/s00216-016-9387-1 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 May;408(12):3233-8. doi: 10.1007/s00216-016-9387-1. Epub 
      2016 Feb 17.