PMID- 26887344
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20231111
IS  - 1600-6143 (Electronic)
IS  - 1600-6135 (Print)
IS  - 1600-6135 (Linking)
VI  - 16
IP  - 8
DP  - 2016 Aug
TI  - Proteomic Characterization Reveals That MMP-3 Correlates With Bronchiolitis 
      Obliterans Syndrome Following Allogeneic Hematopoietic Cell and Lung 
      Transplantation.
PG  - 2342-51
LID - 10.1111/ajt.13750 [doi]
AB  - Improved diagnostic methods are needed for bronchiolitis obliterans syndrome 
      (BOS), a serious complication after allogeneic hematopoietic cell transplantation 
      (HCT) and lung transplantation. For protein candidate discovery, we compared 
      plasma pools from HCT transplantation recipients with BOS at onset (n = 12), 
      pulmonary infection (n = 16), chronic graft-versus-host disease without pulmonary 
      involvement (n = 15) and no chronic complications after HCT (n = 15). Pools were 
      labeled with different tags (isobaric tags for relative and absolute 
      quantification), and two software tools identified differentially expressed 
      proteins (>/=1.5-fold change). Candidate proteins were further selected using a 
      six-step computational biology approach. The diagnostic value of the lead 
      candidate, matrix metalloproteinase 3 (MMP3), was evaluated by enzyme-linked 
      immunosorbent assay in plasma of a verification cohort (n = 112) with and without 
      BOS following HCT (n = 76) or lung transplantation (n = 36). MMP3 plasma 
      concentrations differed significantly between patients with and without BOS (area 
      under the receiver operating characteristic curve 0.77). Consequently, MMP3 
      represents a potential noninvasive blood test for diagnosis of BOS.
CI  - (c) Copyright 2016 The American Society of Transplantation and the American Society 
      of Transplant Surgeons.
FAU - Liu, X
AU  - Liu X
AD  - Department of BioHealth Informatics, Indiana University School of Informatics and 
      Computing, Indianapolis, IN.
FAU - Yue, Z
AU  - Yue Z
AD  - Department of BioHealth Informatics, Indiana University School of Informatics and 
      Computing, Indianapolis, IN.
FAU - Yu, J
AU  - Yu J
AD  - Department of Pediatrics, Indiana University School of Medicine, Indianapolis, 
      IN.
AD  - Herman B Wells Center for Pediatric Research, Indiana University School of 
      Medicine, Indianapolis, IN.
AD  - Department of Microbiology & Immunology, Indiana University School of Medicine, 
      Indianapolis, IN.
AD  - Indiana University Simon Cancer Center, Indiana University School of Medicine, 
      Indianapolis, IN.
FAU - Daguindau, E
AU  - Daguindau E
AD  - Department of Pediatrics, Indiana University School of Medicine, Indianapolis, 
      IN.
AD  - Herman B Wells Center for Pediatric Research, Indiana University School of 
      Medicine, Indianapolis, IN.
AD  - Department of Microbiology & Immunology, Indiana University School of Medicine, 
      Indianapolis, IN.
AD  - Indiana University Simon Cancer Center, Indiana University School of Medicine, 
      Indianapolis, IN.
FAU - Kushekhar, K
AU  - Kushekhar K
AD  - Department of Pediatrics, Indiana University School of Medicine, Indianapolis, 
      IN.
AD  - Herman B Wells Center for Pediatric Research, Indiana University School of 
      Medicine, Indianapolis, IN.
AD  - Department of Microbiology & Immunology, Indiana University School of Medicine, 
      Indianapolis, IN.
AD  - Indiana University Simon Cancer Center, Indiana University School of Medicine, 
      Indianapolis, IN.
FAU - Zhang, Q
AU  - Zhang Q
AD  - Proteomics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA.
FAU - Ogata, Y
AU  - Ogata Y
AD  - Proteomics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA.
FAU - Gafken, P R
AU  - Gafken PR
AD  - Proteomics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA.
FAU - Inamoto, Y
AU  - Inamoto Y
AD  - Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA.
AD  - Division of Hematopoietic Stem Cell Transplantation, National Cancer Center 
      Hospital, Tokyo, Japan.
FAU - Gracon, A
AU  - Gracon A
AD  - Pulmonary Division, Indiana University School of Medicine, Indianapolis, IN.
FAU - Wilkes, D S
AU  - Wilkes DS
AD  - Pulmonary Division, Indiana University School of Medicine, Indianapolis, IN.
FAU - Hansen, J A
AU  - Hansen JA
AD  - Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA.
AD  - University of Washington School of Medicine, Seattle, WA.
FAU - Lee, S J
AU  - Lee SJ
AD  - Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA.
AD  - University of Washington School of Medicine, Seattle, WA.
FAU - Chen, J Y
AU  - Chen JY
AD  - Department of BioHealth Informatics, Indiana University School of Informatics and 
      Computing, Indianapolis, IN.
FAU - Paczesny, S
AU  - Paczesny S
AD  - Department of Pediatrics, Indiana University School of Medicine, Indianapolis, 
      IN.
AD  - Herman B Wells Center for Pediatric Research, Indiana University School of 
      Medicine, Indianapolis, IN.
AD  - Department of Microbiology & Immunology, Indiana University School of Medicine, 
      Indianapolis, IN.
AD  - Indiana University Simon Cancer Center, Indiana University School of Medicine, 
      Indianapolis, IN.
LA  - eng
GR  - R01 HL094260/HL/NHLBI NIH HHS/United States
GR  - P30 CA015704/CA/NCI NIH HHS/United States
GR  - UL1 TR001108/TR/NCATS NIH HHS/United States
GR  - R01 CA118953/CA/NCI NIH HHS/United States
GR  - R01 CA168814/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160314
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
RN  - 0 (Biomarkers)
RN  - 0 (Proteome)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/*blood
MH  - Bronchiolitis Obliterans/blood/*diagnosis/etiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Follow-Up Studies
MH  - Graft vs Host Disease/blood/*diagnosis/etiology
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Humans
MH  - Lung Transplantation/*adverse effects
MH  - Male
MH  - Matrix Metalloproteinase 3/*blood
MH  - Middle Aged
MH  - Prognosis
MH  - Proteome/*analysis
MH  - Proteomics/methods
MH  - Transplantation, Homologous
MH  - Young Adult
PMC - PMC4956556
MID - NIHMS760367
OTO - NOTNLM
OT  - basic (laboratory) research/science
OT  - biomarker
OT  - bone marrow/hematopoietic stem cell transplantation
OT  - bronchiolitis obliterans (BOS)
OT  - lung transplantation/pulmonology
OT  - translational research/science
COIS- Disclosure The authors of this manuscript have conflicts of interest to disclose 
      as described by the American Journal of Transplantation. S.P. is an inventor on a 
      patent on "Methods of detection of graft-versus-host disease" (US- 13/573,766). 
      The other authors have no conflicts of interest to disclose.
EDAT- 2016/02/19 06:00
MHDA- 2017/11/29 06:00
PMCR- 2017/08/01
CRDT- 2016/02/19 06:00
PHST- 2015/10/02 00:00 [received]
PHST- 2016/02/03 00:00 [revised]
PHST- 2016/02/05 00:00 [accepted]
PHST- 2016/02/19 06:00 [entrez]
PHST- 2016/02/19 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - S1600-6135(22)00959-5 [pii]
AID - 10.1111/ajt.13750 [doi]
PST - ppublish
SO  - Am J Transplant. 2016 Aug;16(8):2342-51. doi: 10.1111/ajt.13750. Epub 2016 Mar 
      14.