PMID- 26888507
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20181202
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 44
IP  - 12
DP  - 2015 Dec
TI  - [Clinicopathologic features of embryonal tumor with multilayered rosettes and 
      gene analysis on chromosome 19q13.42].
PG  - 889-94
AB  - OBJECTIVE: To investigate the clinicopathologic features and 19q13.42 gene 
      changes in embryonal tumors with multilayered rosettes (ETMR). METHODS: 
      Immunohistochemistry and fluorescence in situ hybridization (FISH) were performed 
      in three ETMRs. RESULTS: The average age of the patients were 34 months. Imaging 
      revealed huge masses with inhomogeneous enhancement and two cases showed cystic 
      lesions. Follow-up data showed 14 and 38 months survival in two children, the 
      third had a recurrence 4 months after operation. Morphologically, the tumor was 
      mainly composed of dense small primitive neuroepithelial cells in patchy or 
      multilayer rosettes within a background of advanced neuronal differentiation, 
      containing neurocytes, ganglion cells, and neuropil-like background. 
      Immunohistochemical staining showed the neuronal marker, synaptophysin, was 
      positive in differentiated areas. Nestin as a neural stem cell marker was 
      immunoreactive in the primitive neuroepithelial cells including multilayered 
      rosettes. Neurons with positive expression of NeuN were observed occasionally. 
      Ki-67 index was up to 40%-80% in the undifferentiated cells and rosettes, but was 
      only 1%-3% in the differentiated areas. CD99 was positive in perivascular 
      papillary pattern areas in one case. 19q13.42 amplification was detected in more 
      than 30% of tumor cells in all cases. CONCLUSIONS: ETMR is a unique entity with 
      distinctive clinical and pathological features. Chromosome 19q13.42 abnormality 
      is valuable for confirming the diagnosis and for further treatment research.
FAU - Wang, Junmei
AU  - Wang J
AD  - Department of Neuropathology, Beijing Neurosurgical Institute, Capital Medical 
      University, Beijing 100050, China; E-mail: wwwjjjmmm1180@sina.com.
FAU - Liu, Zhaoxia
AU  - Liu Z
FAU - Fang, Jingyi
AU  - Fang J
FAU - Chen, Qian
AU  - Chen Q
FAU - Du, Jiang
AU  - Du J
FAU - Xu, Li
AU  - Xu L
FAU - Li, Guilin
AU  - Li G
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Synaptophysin)
RN  - 0 (neuronal nuclear antigen NeuN, human)
SB  - IM
MH  - Antigens, Nuclear/genetics
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Genetic Testing
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Recurrence, Local
MH  - Neoplasms, Germ Cell and Embryonal/*genetics/*pathology
MH  - Nerve Tissue Proteins/genetics
MH  - Neuropil/pathology
MH  - Synaptophysin/genetics
EDAT- 2016/02/19 06:00
MHDA- 2016/07/07 06:00
CRDT- 2016/02/19 06:00
PHST- 2016/02/19 06:00 [entrez]
PHST- 2016/02/19 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2015 Dec;44(12):889-94.