PMID- 26888843
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20181202
IS  - 0253-3758 (Print)
IS  - 0253-3758 (Linking)
VI  - 43
IP  - 12
DP  - 2015 Dec
TI  - [Culture effects of mouse induced pluripotent stem cells by three-dimensional 
      films in vitro].
PG  - 1073-7
AB  - OBJECTIVE: To observe the proliferation and differentiation capacities of mouse 
      induced pluripotent stem cells (miPSCs) cultured in 
      3-hydroxybutyrate-co-3-hydroxyhexanoate(PHBHHx) three-dimensional films 
      three-dimension films for the purpose of finding a suitable polymeric 
      biomaterials for forming myocardial patches. METHODS: miPSCs were recovered, 
      passaged, cultured and identified, then miPSCs divided into the experimental and 
      control groups. MiPSCs in the experimental group were cultured with PHBHHx 
      three-dimension films. MiPSCs in the control group were cultured with traditional 
      culture dish. Stem cell culture medium or differential medium were added to 
      miPSCs to detecte cell vitality by CCK-8 after 72 hours or to measure the cTnT 
      expression of miPSCs through immunofluorescence or the cTnT expression quantity 
      through flow cytometry after 15 days. RESULTS: Cell activity assay showed that 
      the absorbance values were 0.836 +/- 0.038 in the experimental group, 0.312 +/- 0.004 
      in the control group (P<0.05). Scanning electron microscope (SEM) observation 
      showed that miPSCs grew well on the PHBHHx dimensional films with normal shape. 
      Immunofluorescence results demonstrated positive cTnT expression in both groups 
      and flow cytometry measured cTnT expression was (60.32 +/- 1.76)% in the 
      experimental group and (47.54 +/- 1.46)% in the control group (P<0.05). 
      CONCLUSIONS: miPSCs can survive, proliferate and differentiate on PHBHHx 
      dimensional films. miPSCs proliferation and differentiation capacities are 
      significantly higher in PHBHHx three-dimensional films culture compared with the 
      traditional cell culture.
FAU - Xu, Shijun
AU  - Xu S
AD  - Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen 
      Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 
      100029, China.
FAU - Mu, Junsheng
AU  - Mu J
AD  - Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen 
      Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 
      100029, China; Email: wesleymu@hotmail.com.
FAU - Zhang, Jianqun
AU  - Zhang J
AD  - Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen 
      Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 
      100029, China.
FAU - Bo, Ping
AU  - Bo P
AD  - Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen 
      Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 
      100029, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xin Xue Guan Bing Za Zhi
JT  - Zhonghua xin xue guan bing za zhi
JID - 7910682
RN  - 0 (Biocompatible Materials)
RN  - 0 (Caproates)
RN  - 10191-24-9 (3-hydroxyhexanoic acid)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid
MH  - Animals
MH  - Biocompatible Materials
MH  - Caproates
MH  - *Cell Culture Techniques
MH  - Cell Differentiation
MH  - *Induced Pluripotent Stem Cells
MH  - Mice
EDAT- 2016/02/19 06:00
MHDA- 2016/09/16 06:00
CRDT- 2016/02/19 06:00
PHST- 2016/02/19 06:00 [entrez]
PHST- 2016/02/19 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Xin Xue Guan Bing Za Zhi. 2015 Dec;43(12):1073-7.